SAB4503754
Anti-phospho-AMPK α (pThr172) antibody produced in rabbit
affinity isolated antibody
Synonym(s):
Anti-AMPK, Anti-AMPK2, Anti-AMPKa2, Anti-PRKAA
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 62 kDa
species reactivity
human, rat, mouse
concentration
~1 mg/mL
technique(s)
ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000
NCBI accession no.
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pThr172)
Gene Information
human ... PRKAA1(5562) , PRKAA2(5563)
General description
AMP-activated protein kinase α (AMPKα) exists in two isoforms α1 and α2. The gene coding for AMPK α1 and AMPKα2 is mapped to human chromosome 5p11-p14 and 1p31 respectively. AMPKα is a 63kDa catalytic subunit of AMPK, involved in regulation of AMP levels. The AMP-activated protein kinase (AMPK) is a ubiquitously expressed cellular energy sensor.
Immunogen
The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172.
Immunogen Range: 140-189
Immunogen Range: 140-189
Application
Anti-phospho-AMPK α (pThr172) antibody produced in rabbit has been used in western blot analysis.
Anti-phospho-AMPK α (pThr172) antibody produced in rabbit has been used in western blotting.
Biochem/physiol Actions
AMP-activated protein kinase is activated by the increased concentration of adenosine mono phosphate (AMP) during mitochondrial dysfunction. AMPK, or AMPK α1, is a vital constituent of the adaptive response to cardiomyocyte stress that occurs during ischemia. AMPK is involved in regulation of various cellular processes such as mitochondrial biogenesis, autophagy and inflammation, and immune responses. AMPK signaling pathway aids in maintaining energy balance in retinal neurons, therefore, it is considered as a potential therapeutic target to prevent or delay retinal degeneration. AMPK acts as a cellular energy sensor that controls glucose and lipid metabolism in response to change in nutrient and intracellular energy levels. C allele of protein kinase AMP-activated catalytic subunit α 1 (PRKAA1) rs13361707 is associated with susceptibility of gastric cancer (GCa). AMPKα2 plays an essential role in controlling postmortem glycolysis in skeletal muscle.
Features and Benefits
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.
Physical form
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Find documentation for the products that you have recently purchased in the Document Library.
The AMPK signalling pathway coordinates cell growth, autophagy and metabolism.
Mihaylova MM and Shaw RJ
Nature Cell Biology, 13(9), 1016-1023 (2011)
Activation of AMPK by metformin promotes renal cancer cell proliferation under glucose deprivation through its interaction with PKM2
Liu M, et al.
International Journal of Biological Sciences, 15(3), 617-617 (2019)
Angiotensin-converting enzyme 2 regulates autophagy in acute lung injury through AMPK/mTOR signaling
Zhang X, et al.
Archives of Biochemistry and Biophysics, 672, 108061-108061 (2019)
AMPK in cardiac fibrosis and repair: Actions beyond metabolic regulation.
Daskalopoulos EP
Journal of Molecular and Cellular Cardiology, 91, 188-200 (2016)
Genetic variant of PRKAA1 and gastric cancer risk in an eastern Chinese population.
Qiu LX
Oncotarget, 6(40), 42661-42666 (2015)
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