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Key Documents

Safety Information

SAB4503001

Sigma-Aldrich

Anti-p53, antibody produced in rabbit

affinity isolated antibody

Synonym(s):

Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, tumor protein p53

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 43 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

ELISA: 1:40000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

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technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

growth mode

adherent or suspension

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

morphology

rod shaped

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

General description

Anti-p53 Antibody detects endogenous levels of total p53 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human p53.

Immunogen Range: 344-393

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    210530

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Ayman I Elkady et al.
    World journal of gastroenterology, 20(41), 15275-15288 (2014-11-12)
    To investigate the effects of extracts from Rhazya stricta (R. stricta) and Zingiber officinale (Z. officinale) on human colorectal cancer cells. Human colorectal cancer cells (HCT116) were subjected to increasing doses of crude alkaloid extracts from R. stricta (CAERS) and
    Biljana Miova et al.
    Journal of cellular biochemistry, 116(10), 2365-2374 (2015-04-11)
    The relevance of heat preconditioning resides in its ability to protect cells from different kinds of injury by induction of heat shock proteins, a process in which the intensity of heat stress (HS) and duration of subsequent recovery are vital.

    Articles

    p53: DNA Damage Response and Cancer

    p53 regulates gene expression, cell cycle control and functions as a tumor suppressor. Inactivation of p53 is closely tied to cancer development.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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