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R7397

Sigma-Aldrich

ProtectRNA RNase Inhibitor 500× Concentrate

RNase inhibitor for in situ hybridization assays

Synonym(s):

Ribonuclease inhibitor ProtectRNA

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30 ML
¥2,153.97

¥2,153.97


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30 ML
¥2,153.97

About This Item

UNSPSC Code:
41106300
NACRES:
NA.52

¥2,153.97


Please contact Customer Service for Availability
New, lower price on this item!

Quality Level

form

liquid

storage temp.

2-8°C

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This Item
T49042851SRE0068
Quality Level

200

Quality Level

200

Quality Level

500

Quality Level

500

form

liquid

form

-

form

solution

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Not recommended in systems where other enzymatic activity is required.

ProtectRNA ribonuclease(RNase) Inhibitor inhibits most nucleic acid binding enzymes such as RNase and DNase, reverse transcriptase, some RNA and DNA polymerases, protein synthesis enzymes, and several restriction endonucleases. In contrast, ProtectRNA does not interfere with DNA and RNA hybridizations. The action ofProtectRNA as a nuclease inhibitor can be used as an advantage for in situ hybridization. ProtectRNA is useful during all steps of in situ hybridization for tissue RNA protection from RNase degradation. It is recommended to add ProtectRNA to all aqueous solutions used, from as early as the deparaffinization step until the hybridization is completed.

Application

A potent inhibitor of most nucleic acid binding enzymes, and thus useful as an RNase inhibitor. Especially useful when performing in situ hybridization. If it is added to all aqueous solutions used, it eliminates the need for special glassware washing and after-wash treatments. The 500x concentrate is economical; 2 ml treats 1,000 ml of solution. Not recommended in systems where other enzymatic activity is required.
ProtectRNA RNase Inhibitor 500× Concentrate has been used in:
  • RNA extraction[1]
  • tissue sectioning and staining for laser capture microscopy[2]
  • the isolation of blood vessels from hippocampi[3]
ProtectRNA RNase Inhibitor 500× Concentrate is an RNase inhibitor.[4][5][6]

Features and Benefits

  • The 500× concentrate is economical
  • Eliminates the need for special dishwashing and“after washing” cleaning and treatments.

Quantity

2mL treats 1,000ml of solution.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Legal Information

ProtectRNA is a trademark of Sigma-Aldrich Co. LLC

Pictograms

FlameCorrosion

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3 - Met. Corr. 1

Storage Class Code

3 - Flammable liquids

WGK

WGK 2

Flash Point(F)

140.0 °F - closed cup

Flash Point(C)

60 °C - closed cup

Regulatory Information

危险化学品

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