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Merck
CN

M1028

氯化镁 溶液

Molecular Biology, 1.00 M±0.01 M

别名:

MgCl2, dichloromagnesium, magnesium chloride

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关于此项目

线性分子式:
MgCl2
化学文摘社编号:
7786-30-3
分子量:
95.21
NACRES:
NA.52
PubChem Substance ID:
24896582
UNSPSC Code:
12352302
MDL number:
MFCD00011106
Grade:
Molecular Biology
Form:
liquid
Solubility:
water: soluble

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产品名称

氯化镁 溶液, Molecular Biology, 1.00 M±0.01 M

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

SMILES string

Cl[Mg]Cl

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

grade

Molecular Biology

sterility

non-sterile; 0.2 μm filtered

form

liquid

concentration

1.00 M±0.01 M

technique(s)

DNA sequencing: suitable

impurities

DNAse, none detected
 NICKase, none detected
 Protease, none detected
 RNAse, none detected

solubility

water: soluble

density

1.080 g/cm3

cation traces

heavy metals (as Pb): ≤5 ppm

suitability

suitable for electrophoresis
suitable for molecular biology

application(s)

cell analysis

foreign activity

DNase, RNase, NICKase and protease, none detected

Quality Level

300

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相关类别

Application

氯化镁在各种分子生物学应用，包括PCR反应和缓冲液中提供镁离子。用途：
  • 配制免疫沉淀实验的细胞裂解缓冲液
  • 加入Krebs-Henseleit缓冲液，用于老年小鼠的长期高脂饮食研究。
  • 用于三维微生物转谷氨酰胺酶水凝胶中的细胞透化，进行骨细胞分化及互连细胞网络的体外研究。
  • 保护肝脏免遭氧化损伤。
  • 制备无血清培养基，用于大鼠脑切片培养物的电生理研究。

Features and Benefits

  • 即用型溶液，免去配制步骤，节约时间。
  • 重金属（以铅计）：≤5 ppm
  • 不含DNA酶、RNA酶、切口酶和蛋白酶

General description

氯化镁是一种常见的离子型卤化物，易溶于水。它在酶学、细胞膜/细胞壁结构、肌肉细胞生理学和核酸结构中起多种重要的生物学作用。此外，氯化镁在许多酶中都是基本的辅酶，包括脱氧核糖核酸酶、某些限制性酶和核糖核酸酶H。氯化镁在各种分子生物学应用中（包括PCR反应）广泛用于提供镁离子。

Other Notes

如需了解生化试剂系列的更多信息，请填写此表。
氯化镁溶液是含有1M氯化镁的18兆欧水。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000506880
0000506880
0000474315
0000474315
0000471820

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实验方案

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Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

Enzymatic Assay of Glucose-6-Phosphate Dehydrogenase (EC 1.1.1.49)

To measure glucose-6-phosphate dehydrogenase activity, beta-nicotinamide adenine dinucleotide phosphate is used in a spectrophotometric rate determination assay at 340 nm.

Enzymatic Assay of Hexokinase

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

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