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Merck
CN

M1028

Sigma-Aldrich

氯化镁 溶液

for molecular biology, 1.00 M±0.01 M

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别名:
MgCl2, dichloromangesium, magnesium chloride
线性分子式:
MgCl2
CAS号:
分子量:
95.21
MDL编号:
UNSPSC代码:
12352302
PubChem化学物质编号:
NACRES:
NA.52

等级

for molecular biology

质量水平

无菌性

non-sterile; 0.2 μm filtered

形式

liquid

浓度

1.00 M±0.01 M

技术

DNA sequencing: suitable

杂质

DNAse, none detected
NICKase, none detected
Protease, none detected
RNAse, none detected

溶解性

water: soluble

密度

1.080 g/cm3

痕量阳离子

heavy metals (as Pb): ≤5 ppm

适用性

suitable for electrophoresis
suitable for molecular biology

应用

cell analysis

异质活性

DNase, RNase, NICKase and protease, none detected

SMILES字符串

Cl[Mg]Cl

InChI

1S/2ClH.Mg/h2*1H;/q;;+2/p-2

InChI key

TWRXJAOTZQYOKJ-UHFFFAOYSA-L

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一般描述

氯化镁是一种常见的离子型卤化物,易溶于水。它在酶学、细胞膜/细胞壁结构、肌肉细胞生理学和核酸结构中起多种重要的生物学作用。此外,氯化镁在许多酶中都是基本的辅酶,包括脱氧核糖核酸酶、某些限制性酶和核糖核酸酶H。氯化镁在各种分子生物学应用中(包括PCR反应)广泛用于提供镁离子。

应用

氯化镁在各种分子生物学应用,包括PCR反应和缓冲液中提供镁离子。用途:
  • 配制免疫沉淀实验的细胞裂解缓冲液
  • 加入Krebs-Henseleit缓冲液,用于老年小鼠的长期高脂饮食研究。
  • 用于三维微生物转谷氨酰胺酶水凝胶中的细胞透化,进行骨细胞分化及互连细胞网络的体外研究。
  • 保护肝脏免遭氧化损伤。
  • 制备无血清培养基,用于大鼠脑切片培养物的电生理研究。

特点和优势

  • 即用型溶液,免去配制步骤,节约时间。
  • 重金属(以铅计):≤5 ppm
  • 不含DNA酶、RNA酶、切口酶和蛋白酶

组分

氯化镁溶液是含有1M氯化镁的18兆欧水。

其他说明

For additional information on our range of Biochemicals, please complete this form.

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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实验方案

在测定变溶菌素活性时,使用了一种在600 nm下的连续分光光度法进行测定。粪链球菌细胞壁被用作酶的底物。

This procedure may be used for Mutanolysin products.

Measure hexokinase activity using a continuous spectrophotometric rate-determination assay at 340 nm, catalyzing D-hexose sugar phosphorylation using ATP.

在测定己糖激酶活性时,使用了一种在340nm处的连续分光光度法进行测定。己糖激酶使用ATP作为磷酸的来源来催化D-己糖的磷酸化。

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