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Safety Information

R4154

Sigma-Aldrich

Anti-RbAp46, N-terminal antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):

Anti-RBBP7, Anti-Retinoblastoma-binding Protein 7

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About This Item

MDL number:
MFCD08705514
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 46 kDa

species reactivity

human

technique(s)

indirect immunofluorescence: 1:200-1:400 using human HeLa cells fixed with paraformaldehyde/Triton
western blot: 1:1,000-1:2,000 μg/mL using nuclear extracts of human HeLa cells

UniProt accession no.

Q16576

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Anti-RbAp46, N-terminal antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

R4154

Anti-RbAp46, N-terminal antibody produced in rabbit

Anti-RbAp48/RbAp46 antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

R3779

Anti-RbAp48/RbAp46 antibody produced in rabbit

Anti-RbAp48 antibody produced in rabbit affinity isolated antibody, buffered aqueous solution

R3654

Anti-RbAp48 antibody produced in rabbit

Anti-Importin 7 (C-terminal) antibody produced in rabbit ~1.0 mg/mL, affinity isolated antibody

SAB4200152

Anti-Importin 7 (C-terminal) antibody produced in rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody form

IgG fraction of antiserum

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

indirect immunofluorescence: 1:200-1:400 using human HeLa cells fixed with paraformaldehyde/Triton, western blot: 1:1,000-1:2,000 μg/mL using nuclear extracts of human HeLa cells

technique(s)

indirect immunofluorescence: 1:500-1:1,000 using human HeLa cells fixed with paraformaldehyde-Triton, microarray: suitable, western blot: 1:2,000-1:4,000 using MCF7 nuclear extracts

technique(s)

indirect immunofluorescence: 1-2 μg/mL using HeLa cells fixed with paraformaldehyde-Triton, western blot: 0.5-1 μg/mL using MCF7 nuclear extracts

technique(s)

immunoprecipitation (IP): 5-10 μg using lysates of human HEK-293 cells, western blot: 1-2 μg/mL using whole extracts of human HeLa cells

Gene Information

human ... RBBP7(5931)

Gene Information

human ... RBBP4(5928), RBBP7(5931)
mouse ... Rbbp4(19646), Rbbp7(245688)
rat ... Rbbp4(313048), Rbbp7(83712)

Gene Information

human ... RBBP4(5928)
mouse ... Rbbp4(19646)
rat ... Rbbp4(313048)

Gene Information

human ... IPO7(10527)

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

General description

Retinoblastoma-associated binding protein 46 (RbAp46), also referred to as retinoblastoma-binding protein 7 (RBBP7) has 89% amino acid identity with RbAp48. This nuclear protein belongs to the WD-repeat protein family. It has four repeats that ends with Trp-Asp(WD) residues. RbAp46 is an important constituent of the histone modifying and remodeling complexes. RbAp46 gene is located on human chromosome Xp22.13

Specificity

Anti-RbAp46, N-terminal specifically recognizes human RbAp46.

Immunogen

synthetic peptide corresponding to amino acids 1-14 of human RbAp46, conjugated to KLH via a C-terminal added cysteine residue. The sequence differs in mice by one amino acid.

Application

Anti-RbAp46, N-terminal antibody produced in rabbit may be used in immunoblotting and immunofluorescence.

Biochem/physiol Actions

Retinoblastoma-associated binding protein 46 (RbAp46) plays an important role in the modification and remodeling of chromatin during cell growth and differentiation. It is associated with the nucleosomal remodeling and deacetylation complex (NURD). It may also play a key role in histone deacetylation and transcription repression.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Lot/Batch Number
    085K4751

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    Find documentation for the products that you have recently purchased in the Document Library.

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    The role of retinoblastoma-associated proteins 46 and 48 in estrogen receptor alpha mediated gene expression
    Creekmore AL, et al.
    Molecular and Cellular Endocrinology, 291(1-2), 79-86 (2008)
    Characterization of the gene encoding mouse retinoblastoma binding protein-7, a component of chromatin-remodeling complexes
    Yang J, et al.
    Genomics, 80(4), 407-415 (2002)
    Rb-associated protein 46 (RbAp46) inhibits transcriptional transactivation mediated by BRCA1
    Chen GC, et al.
    Biochemical and Biophysical Research Communications, 284(2), 507-514 (2001)

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