R3779
Anti-RbAp48/RbAp46 antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-Retinoblastoma-binding protein
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Pricing and availability is not currently available.
Recommended Products
biological source
rabbit
Quality Level
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 46 kDa (RbAp46)
antigen 48 kDa (RbAp48)
species reactivity
human
technique(s)
indirect immunofluorescence: 1:500-1:1,000 using human HeLa cells fixed with paraformaldehyde-Triton
microarray: suitable
western blot: 1:2,000-1:4,000 using MCF7 nuclear extracts
General description
pRB-associated proteins p48 (RbAp48) is a 425 amino acids WD-domain protein isolated as a RB1 (retinoblastoma binding protein). RbAp46 (also known as RBBP7- retinoblastoma-binding protein 7) has 89% amino acid identity with RbAp48.
Immunogen
synthetic peptide corresponding to amino acids 413-425 of human RbAp48, conjugated to KLH via an N-terminal added lysine residue. The immunizing peptide is conserved in human, mouse, and rat.
Application
Anti-RbAp48/RbAp46 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.
Biochem/physiol Actions
RbAp46 and RbAp48 are two histone tail-targeting proteins, that are found in association with chromatin remodeling complexes Sin3A/ histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex). Transcriptional repressors exert their effects by recruitment of the Sin3A/HDAC corepressor complex, which contains a module composed of Sin3A, HDAC1, HDAC2, RbAp46, RbAp48, SAP30, and others. In the NuRD complex, HDAC1, HDAC2, RbAp48, and RbAp46 associate with MTA2 (metastasis associated 1 family member 2), MBD3 (methyl-CpG binding domain protein), MAT1L1, MBD3L1 (methyl-CpG binding domain protein 3 like 1), CHD3, and CHD4 to form the nucleosomal remodeling and deacetylation (NuRD) complex.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
常规特殊物品
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
The strategy of fusion genes construction determines efficient expression of introduced transcription factors.
Adamus T, et al.
Acta Biochimica Polonica, 61(4) (2014)
Identification and Characterization of Three New Components of the mSin3A Corepressor Complex
Fleischer TC, et al.
Molecular and Cellular Biology, 23, 3456-3467 (2003)
Seung-Gi Jin et al.
The Journal of biological chemistry, 280(13), 12700-12709 (2005-02-11)
MBD2 and MBD3 are two proteins that contain methyl-CpG binding domains and have a transcriptional repression function. Both proteins are components of a large CpG-methylated DNA binding complex named MeCP1, which consists of the nucleosome remodeling and histone deacetylase complex
Salomé Manska et al.
Virology, 566, 26-41 (2021-12-04)
Upon entry of Human cytomegalovirus (HCMV) into the host cell, the viral genome is transported to the nucleus where it serves as a template for transcription and genome replication. Production of new viral genomes is a coordinated effort between viral
Shuai Leng et al.
Cell death and differentiation, 28(12), 3329-3343 (2021-06-25)
Pancreatic cancer is a common malignant tumor with poor prognosis. Recently, cancer stem cells (CSCs) were identified in several solid tumors, including pancreatic cancer. Although accumulating evidence indicates that sirtuin 1 (SIRT1) exerts biological functions in various cancers, how it
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service
