PHG0005
BIS-TRIS propane
Synonym(s):
1,3-Bis[tris(hydroxymethyl)methylamino]propane
Sign In to View Organizational & Contract Pricing.
Select a Size
About This Item
Linear Formula:
CH2[CH2NHC(CH2OH)3]2
CAS Number:
Molecular Weight:
282.33
EC Number:
264-899-3
UNSPSC Code:
12161705
NACRES:
NA.21
Beilstein/REAXYS Number:
1786109
MDL number:
InChI
1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2
InChI key
HHKZCCWKTZRCCL-UHFFFAOYSA-N
SMILES string
OCC(CO)(CO)NCCCNC(CO)(CO)CO
biological source
synthetic
form
powder
technique(s)
cell culture | mammalian: suitable
impurities
Endotoxin, microbial, and trace metals; tested
useful pH range
6.3-9.5
pKa (25 °C)
(1) 6.8, (2) 9.0
mp
164-165 °C (lit.)
suitability
suitable for manufacturing use
foreign activity
Cytotoxicity, DNase, NICKase, RNase, and Protease; tested
Quality Level
Looking for similar products? Visit Product Comparison Guide
Application
Bis Tris Propane is a unique biological buffer also referred to as a “Universal” buffer, which means the buffer capacity covers a wide range. Bis Tris Propane has two pKas of 6.3 and 9.5, which expands the buffering range from 5.0-10.5-making this a versatile buffer for biological systems. Bis Tris Propane is considered to be non-toxic to culture cell lines and provides high-solution clarity.
A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.
A review of DNA polymerase techniques lists bis tris propane as a suitable buffer for PCR methods as a universal buffer pH gradient is employed.
General description
Our SAFC® portfolio of high-quality raw materials for use in biopharmaceutical processing withstands strict quality control procedures plus the documentation and expertise to help our customers meet requirements as defined by the M-Clarity Program.
M-Clarity Program
Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.
Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
M-Clarity Program
Buffer quality is vital for the success of biopharmaceutical processes, because buffers are indispensable in nearly every production step.
Our broad portfolio of buffer materials manufactured under appropriate controls is tailored to your needs. Ranging from non-GMP grades for low-risk application, to IPEC-PQG GMP for higher-risk applications, we have products covering all your manufacturing needs.
Legal Information
SAFC is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Natalie Jayne Werling et al.
Human gene therapy methods, 26(3), 82-92 (2015-05-09)
Adeno-associated viral (AAV) vectors show great promise for gene therapy because of their excellent safety profile; however, development of robust dose-determining assays for AAV has presented a significant challenge. With the ultimate goal of future harmonization and standardization of AAV
Francesca Spyrakis et al.
ChemMedChem, 9(7), 1501-1511 (2014-03-13)
Cystalysin from Treponema denticola is a pyridoxal 5'-phosphate dependent lyase that catalyzes the formation of pyruvate, ammonia, and sulfide from cysteine. It is a virulence factor in adult periodontitis because its reaction contributes to hemolysis, which sustains the pathogen. Therefore
Milya Davlieva et al.
Nucleic acids research, 43(9), 4758-4773 (2015-04-22)
LiaR is a 'master regulator' of the cell envelope stress response in enterococci and many other Gram-positive organisms. Mutations to liaR can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. LiaR is phosphorylated in
Roland D Kersten et al.
ACS chemical biology, 10(11), 2501-2511 (2015-09-18)
Terpenes are ubiquitous natural chemicals with diverse biological functions spanning all three domains of life. In specialized metabolism, the active sites of terpene synthases (TPSs) evolve in shape and reactivity to direct the biosynthesis of a myriad of chemotypes for
Frank Jansen et al.
Biotechnology and applied biochemistry, 61(6), 646-654 (2014-03-01)
The plant polymer lignin is the greatest source of aromatic chemical structures on earth. Hence, the chemically diverse lignin monomers are valuable raw materials for fine chemicals, materials synthesis, and food and flavor industries. However, extensive use of this natural
Related Content
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service