P5704
Monoclonal Anti-Protein Kinase C (PKC) antibody produced in mouse
clone MC5, ascites fluid
Synonym(s):
Monoclonal Anti-Protein Kinase C, Anti-PKC
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About This Item
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
MC5, monoclonal
mol wt
antigen 80 kDa
contains
15 mM sodium azide
species reactivity
rat, bovine, mouse, human
technique(s)
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:500 using rat brain cytosol preparation
isotype
IgG2a
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... PRKCB(5579) , PRKCZ(5590)
mouse ... Prkcb(18751) , Prkcc(18752)
rat ... Prkcb(25023) , Prkcg(24681)
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General description
Protein Kinase C (PKC) is a phospholipid and calcium dependent enzyme that regulates intracellular signal transduction. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity, and release of neurotransmitters. PKC inhibitors have been implicated in diabetic retinopathy . Monoclonal Anti-Protein Kinase C (clone MC5) recognizes an epitope located within the amino acid sequence 296-317 at the hinge region (close to or at the trypsin cleavage site) of PKC . The antibody is specific for PKC in humans, bovines, rats and mice. Binding of the antibody to in vitro purified PKC blocks trypsin-mediated partial proteolysis. Phorbol-ester induced down-regulation of the kinase is inhibited by the introduction of Fab fragment of the antibody into a rodent glioma cell line .
Protein kinase C (PKC) is a 77-90kD protein that belongs to serine/threonine protein kinases family. It has a crucial role in cellular regulation, signal transduction and tumor promotion and oncogenesis. Monoclonal Anti-Protein Kinase C (PKC) antibody can be used for identification and localization of PKC in normal and malignant tissues. It can also be used in immunoblotting (diluted 1:500) using rat brain extracts. Mouse Anti-Protein Kinase C (PKC) antibody reacts specifically with PKC polypeptide (80kD). The product has also shown cross reactivity for rat, bovine, mouse and human.
Specificity
Reacts with the 80 kDa polypeptide of PKC. Binding of the antibody to purified kinase in vitro, blocks partial proteolysis by trypsin. Introduction of Fab fragments of the antibody into a rodent glioma cell line inhibits phorbol-ester-induced down-regulation of the kinase.
Immunogen
purified bovine brain protein kinase C
Application
Monoclonal Anti-Protein Kinase C (PKC) antibody can be used for the localization of protein kinase C by using different immunochemical assays like- dot blot, immunoprecipitation and ELISA. It can also be used as a primary antibody in immunohistochemistry (diluted 1:200) .
Monoclonal Anti-Protein Kinase C (PKC) antibody is suitable for use in immunohistochemistry (frozen sections), immunoblot (80 kD, using bovine brain PKC, extracts of rat glioma and murine NIH 3T3 cell lines), dot blot, ELISA, microarray and immunoprecipitation (rat glioma and human cell extracts/lysates).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Certificates of Analysis (COA)
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Zi-Li Yu et al.
International journal of molecular medicine, 37(2), 468-474 (2016-01-09)
Lysophosphatidic acid (LPA) is an efficient, bioactive phospholipid involved in various biological processes. In this study, LPA-induced connective tissue growth factor (CTGF/CCN2) expression and the underlying mechanisms were investigated using the MC3T3-E1 cell line. The MC3T3-E1 cells were stimulated with
Influence of intrathecal injection with dexmedetomidine on the
behavioral ability and analgesic effects on rats with neuropathic
pain and expression of protein kinase C in the spinal dorsal horn
behavioral ability and analgesic effects on rats with neuropathic
pain and expression of protein kinase C in the spinal dorsal horn
Li X and Zhang W
Experimental and Therapeutic Medicine, 16(5), 3835-3840 (2018)
Akira Hara et al.
Stem cells and development, 17(4), 619-627 (2008-04-09)
In vivo transplantation of undifferentiated embryonic stem (ES) cells can produce teratomas with uncontrolled cell proliferation. Although ES cells may be attractive candidates for human cell-replacement therapy in the future, the major limitation of its application to the therapy is
Haruki Takeuchi et al.
BioMed research international, 2018, 2963232-2963232 (2018-06-02)
TRPM1, the first member of the melanoma-related transient receptor potential (TRPM) subfamily, is the visual transduction channel downstream of metabotropic glutamate receptor 6 (mGluR6) on retinal ON bipolar cells (BCs). Human TRPM1 mutations are associated with congenital stationary night blindness
S Young et al.
European journal of biochemistry, 173(1), 247-252 (1988-04-05)
A monoclonal antibody to protein kinase C is described that recognises the site of limited proteolysis on the native enzyme. Binding of the antibody to the purified kinase in vitro blocks partial proteolysis by trypsin, and introduction of the Fab
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