Key Documents
Safety Information
P5147
Protease from Streptomyces griseus
Type XIV, ≥3.5 units/mg solid, powder
Synonym(s):
Actinase E, Pronase E
Select a Size
Select a Size
About This Item
Recommended Products
biological source
Streptomyces griseus
Quality Level
type
Type XIV
form
powder
specific activity
≥3.5 units/mg solid
solubility
10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble 0.2 mg/mL at 37 °C, clear, colorless to tan
shipped in
wet ice
storage temp.
−20°C
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Related Categories
1 of 4
This Item | 603864 | 106631 | T36609 |
|---|---|---|---|
| assay 98% | assay - | assay 98% | assay 99% |
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level 100 |
| bp 274-275 °C (lit.) | bp 274-275 °C | bp 225-227 °C (lit.), 95-96 °C/10 mmHg (lit.) | bp 263 °C (lit.) |
| mp 177-180 °C (lit.) | mp 177-180 °C | mp −4-−2 °C (lit.) | mp 107-113 °C (lit.) |
Specificity
Application
- for the digestion of nucleus pulposus (NP) tissue[1]
- in the catalysis of asymmetric one pot Mannich reaction[2]
- for deproteinization of starch[3]
- digestion of brain slices for the cell dissociation[4]
Biochem/physiol Actions
Features and Benefits
- highly stable in pH range of 5.0 to 9.0, with peak activity at pH 8.8
- compatible with many DNA and RNA isolation buffers
- broad substrate specificity
Quality
Physical properties
Unit Definition
Preparation Note
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
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Certificates of Analysis (COA)
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Is Product P5147, Protease from Streptomyces griseus, also described as "Pronase"?
"Pronase E" is the name given to a group of proteolytic enzymes produced by Streptomyces griseus K-1. At least 10 proteases are in the mixture, five serine-type proteases, two zinc endopeptidases, two zinc leucine aminopeptidases, and one zinc carboxypeptidase.
Does Product P5147, Protease from Streptomyces griseus, contain calcium acetate?
This product contains calcium acetate buffer salt. The amount of calcium acetate present will vary for each lot number and has been observed to range from 2 - 30% (w/w).
How should Product P5147, Protease from Streptomyces griseus, be reconstituted and how should a stock solution be prepared?
This product can be dissolved in 0.01 M sodium acetate with 0.005 M calcium acetate at pH 7.5 at 37°C; at 0.2 mg/mL, this yields a clear solution which ranges from colorless to light tan. Calcium ion is recommended for protection from autolysis. The activity of a dilute enzyme solution containing 0.01 to 0.1 M calcium ion was stable over 24 hours at neutral pH at 2-8°C. Pronase E is stable at 4°C for at least six months. Stock solutions of 5 to 20 mg/mL in water are usually stored at -20°C.
Under what conditions can Product P5147, Protease from Streptomyces griseus, be used to digest protein?
For DNA isolation, Pronase E is usually prepared as a stock solution, and prior to storage at -20°C, the solution is first heated to 56°C for about 15 minutes, then incubated at 37°C for 1 hour. This encourages self-digestion, to eliminate DNAse and RNAse contamination. The enzyme is added to a DNA sample (in the presence of 0.5-1%(w/v) SDS to disrupt DNA protein interactions) typically at 250-500 μg protein/mL, then incubated at 37°C for 1-4 hours. For protein hydrolysis, dissolve about 0.2 μmol of protein in 0.2 mL of 50 mM ammonium bicarbonate buffer at pH 8 (or phosphate buffer pH 7). Add Pronase to 1% (w/w) and incubate at 37°C for 24 hours. It may be necessary to add aminopeptidase M at 4% (w/w) and incubate at 37°C for another 18 hours.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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