Skip to Content
Merck
CN
All Photos(8)

Documents

Safety Information

H3149

Sigma-Aldrich

Heparin sodium salt from porcine intestinal mucosa

Grade I-A, ≥180 USP units/mg, powder, BioReagent, suitable for cell culture

Sign Into View Organizational & Contract Pricing
All Photos(8)

About This Item

CAS Number:
9041-08-1
MDL number:
MFCD00081689
UNSPSC Code:
12352201
NACRES:
NA.21

biological source

Porcine intestinal mucosa

Quality Level

200

type

Grade I-A

product line

BioReagent

form

powder

specific activity

≥180 USP units/mg

technique(s)

analytical sample preparation: suitable
cell culture | mammalian: suitable

color

beige

solubility

H2O: 50 mg/mL

application(s)

cell analysis
diagnostic assay manufacturing
life science and biopharma
sample preparation

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Heparin is mainly responsible for the delay in the coagulation of blood. It enhances the antithrombin-mediated inactivation of proteases in the coagulation pathway.
Heparin sodium, a sulfated polysaccharide from the glycosaminoglycan family, plays a vital role in cell culture, cancer biology, and drug discovery research. Its impact on protein and cell interactions makes it valuable for studying molecular and cellular processes, tumor modeling, exploring binding interactions, and as a cell culture supplement to support cell viability.

Application

  • Heparin sodium salt from porcine intestinal mucosa has been used in sLRP (soluble circulating low density lipoprotein receptor-related protein-1) purification from human plasma.
  • It has been used as a supplement in M199 medium for culturing of human umbilical vein endothelial cells.
  • It has been used as an anticoagulant agent during isolation of blood.

Features and Benefits

  • High-purity compound suitable for a wide variety of research applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.
To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

  1. How is Heparin sodium salt from porcine intestinal mucosa, Product H3149, solubilized?

    Heparin sodium salt from porcine intestinal mucosa is soluble in deionized water at a concentrationi of 50 mg/mL.

  2. How much of Heparin sodium salt from porcine intestinal mucosa, Product H3149, is used for cell culture?

    Solutions at 0.5 mg per mL were tested in cell culture. Start at this concentration, and titer from there to determine the optimal concentration for usage.

  3. How are solutions Heparin sodium salt from porcine intestinal mucosa, Product H3149, stored?

    Solutions can be stored at 2-8C for up to 2 years, if sterile filtered through a 0.22 micron filter. Benzyl alcohol (0.06-1%) can be added to solutions to prevent microbial growth.

  4. How many milligrams of Heparin sodium salt, Product H3149, are in the bottle?

    The potency, in units/mg, is lot-specific; the value can be found on the certificate of analysis. It is typically over 140 units per mg. The number of milligrams can be calculated by dividing the number of units by the potency in units per mg.

  5. What is the molecular weight of Heparin sodium salt?

    Heparin is a mixture of polyanion chains having molecular weights ranging from 6,000 to 30,000 Daltons, with most chains in the range of 17,000 to 19,000 Daltons.

  6. How much Heparin sodium salt, is used to prevent coagulation?

    We suggest using 20 to 50 units per mL of blood.

  7. What is the best way to sterilize Heparin sodium salt?

    Although solutions may be autoclaved at 121 °C for 5 to 10 minutes, we generally recommend sterile filtration. This is because prolonged autoclaving may have an effect on its activity.  

  8. What concentration of Heparin sodium salt, can I use to inhibit RNAse activity?

    Heparin has been used at concentrations ranging from 100 to 500 μg/mL. This has been reported in the J. Biol. Chem., 248, 2095 (1973).

  9. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  10. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  11. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  12. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  13. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Mohamed Abdouh et al.
Journal of experimental & clinical cancer research : CR, 36(1), 113-113 (2017-09-01)
Horizontal transfer of malignant traits from the primary tumor to distant organs, through blood circulating factors, has recently become a thoroughly studied metastatic pathway to explain cancer dissemination. Recently, we reported that oncosuppressor gene-mutated human cells undergo malignant transformation when
Factor Xa and thrombin, but not factor VIIa, elicit specific cellular responses in dermal fibroblasts.
Bachli EB et al.
Journal of Thrombosis and Haemostasis, 1, 1935-1935 (2003)
Ko-Hsun Liao et al.
PloS one, 12(6), e0179758-e0179758 (2017-06-24)
Tissue angiogenesis is intimately regulated during embryogenesis and postnatal development. Defected angiogenesis contributes to aberrant development and is the main complication associated with ischemia-related diseases. We previously identified the increased expression of RUNX1T1 in umbilical cord blood-derived endothelial colony-forming cells
Structure of the antithrombin-binding site in heparin.
Lindahl U et al.
Proceedings of the National Academy of Sciences of the USA, 76, 3198-3202 (1979)
Yin Xiang Setoh et al.
Nature microbiology, 4(5), 876-887 (2019-03-20)
Arboviruses cycle between, and replicate in, both invertebrate and vertebrate hosts, which for Zika virus (ZIKV) involves Aedes mosquitoes and primates1. The viral determinants required for replication in such obligate hosts are under strong purifying selection during natural virus evolution
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service