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P0082
Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse
~1.0 mg/mL, clone PF-29, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-OCT3, Anti-OCT4, Anti-OTF3, Anti-POU domain, class 5, transcription factor 1
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100 μL
¥5,074.24
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100 μL
¥5,074.24
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
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![Anti-OCT-4 [POU5F1] Antibody, clone 7F9.2 clone 7F9.2, from mouse](/deepweb/assets/sigmaaldrich/product/images/307/874/7354f72d-80ee-40a5-b7fa-0590fe6784cc/640/7354f72d-80ee-40a5-b7fa-0590fe6784cc.jpg)
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PF-29, monoclonal
form
buffered aqueous solution
mol wt
antigen ~43 kDa
species reactivity
human
concentration
~1.0 mg/mL
1 of 4
This Item | SMB00702 | 10798 | 178278 |
|---|---|---|---|
| technique(s) HPLC: suitable, gas chromatography (GC): suitable | technique(s) - | technique(s) - | technique(s) - |
| application(s) food and beverages | application(s) metabolomics | application(s) - | application(s) - |
| format neat | format - | format - | format - |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| grade analytical standard | grade - | grade - | grade - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
General description
Monoclonal Anti-POU5F1 (Oct4) (mouse IgG2a isotype) is derived from the hybridoma PF-29 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice, immunized with a peptide.
POU5F1 (POU class 5 homeobox 1) gene codes for a transcription factor that binds to octamer sequence. POU5F1 is mapped to human chromosome 6p21.33.
Specificity
Monoclonal Anti-POU5F1 (Oct4) recognizes human POU5F1/Oct4.
Immunogen
amino acids 20-37 of human POU5F1 peptide.
Application
Monoclonal Anti-POU5F1 (Oct4) antibody produced in mouse may be used in:
- immunoblotting
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
POU5F1 (POU class 5 homeobox 1) controls homeostasis of embryonic cell development and pluripotency. The POU5F1 gene is highly expressed in cancer cells and offers resistance against chemotherapy.
POU5F1 protein functions as an early developmental control gene. This protein has been recognized as fundamental in the maintenance of pluripotency of embryonic stem cells and primordial germ cells. POU5F1 protein has been proposed as a useful marker for germ cell tumors (GCTs) that can exhibit pluripotential, specifically seminoma/ dysgerminoma/germinoma and embryonal carcinoma (EC). POU5F1 has also been established as a marker for extragonadal germ cell tumors (GCT) and metastatic tumors.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Ming-Tse Sung et al.
Human pathology, 37(6), 662-667 (2006-05-31)
Correctly diagnosing a metastatic germ cell tumor after chemotherapy may be challenging because of the diverse morphological manifestations of postchemotherapy tumors. Both OCT4 and CD30 are sensitive markers for the identification of primary embryonal carcinomas; however, loss of expression of
Chemotherapeutics-induced Oct4 expression contributes to drug resistance and tumor recurrence in bladder cancer.
Lu C S, et al.
Oncotarget, 8(19), 30844-30844 (2017)
Timothy D Jones et al.
The American journal of surgical pathology, 28(7), 935-940 (2004-06-30)
OCT4 (POU5F1) is a transcription factor expressed in embryonic stem and germ cells and is involved in the regulation and maintenance of pluripotency. It has been detected in primary testicular germ cell tumors with pluripotent potential, seminoma, and embryonal carcinoma.
Clinical significance of putative markers of cancer stem cells in gastric cancer: A retrospective cohort study.
Chen X L, et al.
Oncotarget, 7(38), 62049-62049 (2016)
Minimum regions of genomic imbalance in stage I testicular embryonal carcinoma and association of 22q loss with relapse.
Gilbert D C, et al.
Genes Chromosomes Cancer, 50(3), 186-195 (2011)
Articles
Quantitative and qualitative western blotting to validate knockdown by esiRNA.
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