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Key Documents

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Safety Information

MAK008

Sigma-Aldrich

Hydroxyproline Assay Kit

sufficient for 100 colorimetric tests

Synonym(s):

Hydroxyproline Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric tests

detection method

colorimetric

relevant disease(s)

dermatological diseases

shipped in

wet ice

storage temp.

2-8°C

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Hydroxyproline Assay Kit sufficient for 100 colorimetric tests

MAK008

Hydroxyproline Assay Kit

Hydroxyproline Assay Kit (Perchlorate-Free) sufficient for 100 colorimetric tests

MAK357

Hydroxyproline Assay Kit (Perchlorate-Free)

Collagen Assay Kit sufficient for 100 fluorometric tests

MAK322

Collagen Assay Kit

Alanine Assay Kit sufficient for 100 colorimetric or fluorometric tests

MAK001

Alanine Assay Kit

detection method

colorimetric

detection method

colorimetric

detection method

fluorometric

detection method

colorimetric, fluorometric

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

dermatological diseases

relevant disease(s)

rheumatological diseases; cancer; neurological disorders; cardiovascular diseases

relevant disease(s)

rheumatological diseases; dermatological diseases; gastrointestinal diseases; pulmonary disorders; cancer

relevant disease(s)

-

General description

Hydroxyproline (4-hydroxyproline) is a non-proteinogenic amino acid[1] formed by the post-translational hydroxylation of proline. Hydroxyproline is a major component of collagen, where it serves to stabilize the helical structure.[2] Because hydroxyproline is largely restricted to collagen, the measurement of hydroxylproline levels can be used as an indicator of collagen content. Conditions that increase collagen turnover can elevate serum and urine hydroxyproline levels. Urine and serum hydroxyproline levels can be used as a marker for bone resorption.[3][4]

Application

Hydroxyproline Assay Kit has been used to determine the concentration of hydroxyproline and collagen.[5][6][7]

Suitability

Suitable for hydroxyproline detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

In the Hydroxyproline Assay Kit, hydroxyproline concentration is determined by the reaction of oxidized hydroxyproline with 4-(Dimethylamino)benzaldehyde (DMAB), which results in a colorimetric (560 nm) product, proportional to the hydroxyproline present.

replaced by

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Eye Dam. 1 - Flam. Liq. 2 - Met. Corr. 1 - Ox. Liq. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system, Thyroid

Supplementary Hazards

EUH031

Storage Class Code

5.1B - Oxidizing hazardous materials

Flash Point(F)

64.4 °F

Flash Point(C)

18 °C

Regulatory Information

危险化学品
易制爆化学品
  • Specification Sheet

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    Certificates of Analysis (COA)

    Lot/Batch Number
    8L15K05550
    8L14K05550
    8J06K05550
    7M14K05550
    8H15K05550

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. Can Product MAK008, Hydroxyproline Assay Kit, be used to measure the hydroxyproline levels in cell culture supernatants?

      Yes.The manufacturer of the kit recommends that cell culture media (that is, cell culture supernatants) should be treated the same as serum or urine samples.

    6. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Proline and hydroxyproline metabolism: implications for animal and human nutrition.
    Wu G, et al.
    Amino Acids, 40(4), 1053-1063 (2011)
    M K North et al.
    Meat science, 110, 145-152 (2015-08-01)
    This study aimed to determine the optimum ageing period for vacuum-packed springbok (Antidorcas marsupialis) Longissimus thoracis et lumborum (LTL) and Biceps femoris (BF) muscle stored at 5.4 ± 1.0°C. Portions of muscle from seven male and six female springbok were
    Krysta Powers et al.
    The Journal of experimental biology, 220(Pt 17), 3110-3118 (2017-06-24)
    In actively stretched skeletal muscle sarcomeres, titin-based force is enhanced, increasing the stiffness of active sarcomeres. Titin force enhancement in sarcomeres is vastly reduced in
    Urinary biochemical markers for bone resorption during the menstrual cycle.
    Gorai I, et al.
    Calcified Tissue International, 57(2), 100-104 (1995)
    Lodish H, et al.
    Molecular Cell Biology. 4th edition. (2000)
    View All Related Papers

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