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Merck
CN

PHR1939

Hydroxyproline

Pharmaceutical Secondary Standard; Certified Reference Material

Synonym(s):

trans-4-Hydroxy-L-proline, (2S,4R)-4-Hydroxypyrrolidine-2-carboxylic acid, Hyp

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About This Item

Empirical Formula (Hill Notation):
C5H9NO3
CAS Number:
51-35-4
Molecular Weight:
131.13
EC Number:
200-091-9
UNSPSC Code:
12352209
NACRES:
NA.24
Beilstein/REAXYS Number:
81441
MDL number:
MFCD00064320

Key Documents

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Product Name

Hydroxyproline, Pharmaceutical Secondary Standard; Certified Reference Material

InChI

1S/C5H9NO3/c7-3-1-4(5(8)9)6-2-3/h3-4,6-7H,1-2H2,(H,8,9)/t3-,4+/m1/s1

InChI key

PMMYEEVYMWASQN-DMTCNVIQSA-N

SMILES string

O[C@H]1CN[C@@H](C1)C(O)=O

grade

certified reference material
pharmaceutical secondary standard

API family

proline

CofA

current certificate can be downloaded

packaging

pkg of 500 mg

mp

273 °C (dec.) (lit.)

application(s)

pharmaceutical

format

neat

storage temp.

2-30°C

Quality Level

300

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Analysis Note

These secondary standards offer multi-traceability to the USP and EP primary standards, where they are available.

Application

This pharmaceutical secondary standard can also be used as follows:

  • Multi-analysis of acetylcarnitine, proline, hydroxyproline, citrulline, and arginine as possible biomarkers by liquid chromatography-tandem mass spectrometry (LC-MS/MS) in human plasma samples
  • Simultaneous determination of proline and hydroxyproline in plasma samples by (LC-MS/MS using a new fluorescent reagent for pre-column derivatization
  • Development of a micellar electrokinetic chromatography (MEKC) method to measure hydroxyproline in various dairy products upon in-capillary derivatization
  • Quantitative analysis of free prolyl dipeptides, proline, and hydroxyproline by flow-gated capillary electrophoresis combined with laser-induced fluorescence detector from unhydrolyzed urine samples

General description

This Certified Reference Material (CRM) is produced and certified in accordance with ISO 17034 and ISO/IEC 17025. All information regarding the use of this CRM can be found on the certificate of analysis.

Pharmaceutical secondary standards for application in quality control provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.

Hydroxyproline, found exclusively in collagen, is formed from the hydroxylation of proline. It is used as a biomarker to measure the collagen content of various biological samples.

Other Notes

Find a digital Reference Material for this product available on our online platform ChemisTwin® for NMR. You can use this digital equivalent on ChemisTwin® for your sample identity confirmation and compound quantification (with digital external standard). An NMR spectrum of this substance can be viewed and an online comparison against your sample can be performed with a few mouseclicks. Learn more here and start your free trial.
Natural constituent of animal structural proteins such as collagen and elastin.
To see an example of a Certificate of Analysis for this material enter LRAC2898 in the Documents slot below. This is an example certificate only and may not be the lot that you receive.

Storage Class

11 - Combustible Solids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Ning Zhang et al.
Analytical and bioanalytical chemistry, 409(30), 7077-7085 (2017-10-04)
Unhydrolyzed prolyl hydroxyproline (Pro-Hyp) and total 4-hydroxyproline (Hyp) in urine have been suggested as disease biomarkers for bone turnover and osteoporosis. Here, a rapid method was developed to accurately and selectively determine free prolyl compounds in unhydrolyzed urine samples. Urine

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