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MAK001

Sigma-Aldrich

Alanine Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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50 PKG
¥2,295.87

¥2,295.87

Estimated to ship on2025年5月19日Details

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50 PKG
¥2,295.87

About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

¥2,295.87

Estimated to ship on2025年5月19日Details

Request a Bulk Order

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

storage temp.

−20°C

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This Item
SLVVR33RSSLGVR33RSSLHVR13SL
Millex™ PVDF针式过滤器 pore size 0.45 μm, diam. 33 mm, sterile, hydrophilic

SLHVR33RS

Millex™ PVDF针式过滤器

Millex™ PVDF 针式过滤器 pore size 0.1 μm, diam. 33 mm, sterile, hydrophilic

SLVVR33RS

Millex™ PVDF 针式过滤器

Millex™ PVDF 针式过滤器 pore size 0.22 μm, diam. 33 mm, sterile, hydrophilic

SLGVR33RS

Millex™ PVDF 针式过滤器

Millex™ PVDF 针式过滤器 pore size 0.45 μm, diam. 13 mm, sterile, hydrophilic

SLHVR13SL

Millex™ PVDF 针式过滤器

manufacturer/tradename

Millex® SLHVR33RS

manufacturer/tradename

Millex® SLVVR33RS

manufacturer/tradename

Millex® SLGVR33RS

manufacturer/tradename

Millex® SLHVR13SL

material

PVDF membrane, modified acrylic housing

material

PVDF membrane, modified acrylic housing

material

PVDF membrane, modified acrylic housing

material

PVDF membrane, high-density polyethylene housing

pore size

0.45 μm

pore size

0.1 μm

pore size

0.22 μm

pore size

0.45 μm

fitting

female Luer-Lok® inlet, male Luer outlet slip

fitting

female Luer-Lok® inlet fitting, male Luer outlet slip

fitting

female Luer-Lok® inlet fitting, male Luer outlet slip

fitting

female Luer-Lok® inlet fitting, male Luer outlet slip

sterility

γ-irradiated, sterile

sterility

γ-irradiated, sterile

sterility

γ-irradiated, sterile

sterility

ethylene oxide treated, sterile

General description

Alanine is a nonessential amino acid that plays a key role in the glucose-alanine cycle between muscle tissue and the liver.[1] In amino acid-degrading tissues such as muscle, amino groups are pooled as glutamate by transamination reactions. The amino group of glutamate is transferred to pyruvate via alanine aminotransferase, forming alanine and α-ketoglutarate. The alanine is passed into the blood and transported to the liver.[2] This reaction is reversed in the liver where pyruvate can be used in gluconeogenesis to form glucose,[3] which may return to other tissues through the circulatory system. Increased alanine levels correlate with higher blood pressure, energy intake, cholesterol levels, and body mass index.

Application

Alanine Assay Kit has been used to determine the intracellular alanine pool.[4]

Suitability

Suitable for alanine detection in cell and tissue culture supernatants, urine, plasma, serum, and other biological samples.

Principle

In this kit, alanine concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/fluorometric (λex = 535/λem = 587 nm) product, proportional to the alanine present. Typical detection ranges for this kit are 2-10 nmole (colorimetric) and 0.2-1 nmole (fluorometric).

replaced by

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Regulatory Information

常规特殊物品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
8F10K06520
8B21K06520
7K22K06520
7F14K06520
7D07K06520

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GABA transaminases from Saccharomyces cerevisiae and Arabidopsis thaliana complement function in cytosol and mitochondria.
Cao J, et al.
Yeast, 30(7), 279-289 (2013)
Dmitry Frank et al.
Neuropharmacology, 155, 173-184 (2019-06-04)
Post-stroke depression (PSD) is a common and serious complication following stroke. Both stroke and depression have independently been associated with pathologically elevated glutamate levels in the brain's extra-cerebral fluid (ECF). Here we evaluate an alternative therapeutic approach to PSD with
Berg J M, et al.
Biochemistry (5th Edition) (2002)
Mauro D P.
Amino Acids and Proteins for the Athlete: The Anabolic Edge (1997)
The complex role of branched chain amino acids in diabetes and cancer.
O'Connell T M.
Metabolites, 3(4), 931-945 (2013)
View All Related Papers

Articles

Glutamine Metabolism in Cancer Cells

Sigma article discusses tumor cell metabolic pathways, focusing on aerobic glycolysis and mitochondrial activity.

Protocols

Enzymatic Method for Determining Alanine (Alanine Assay)

Assay protocol for the colorimetric/fluorometric detection of Alanine in biological samples using the alanine assay kit.

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