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Merck
CN

M5639

Sigma-Aldrich

4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside

glycanase substrate, fluorogenic, ≥98.00% (TLC), powder

Synonym(s):

4-Methyl-7-coumarinyl-tri-N-acetyl-β-chitotrioside, 4-Methylumbelliferyl-N,N′,N″-triacetyl-β-chitotrioside

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25 μL
CN¥2,257.75
100 μL
CN¥4,582.18
200 μL
CN¥5,503.01

CN¥2,257.75


Available to ship onApril 27, 2025Details



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25 μL
CN¥2,257.75
100 μL
CN¥4,582.18
200 μL
CN¥5,503.01

About This Item

Empirical Formula (Hill Notation):
C34H47N3O18
CAS Number:
Molecular Weight:
785.75
Beilstein:
5710839
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

CN¥2,257.75


Available to ship onApril 27, 2025Details


Product Name

4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside, fluorogenic glycanase substrate

Quality Level

Assay

≥98.00% (TLC)

form

powder

solubility

water: pyridine, 1:1: 4.90-5.10 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

SMILES string

CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@H]2[C@H](O)[C@@H](NC(C)=O)[C@@H](O[C@@H]2CO)O[C@H]3[C@H](O)[C@@H](NC(C)=O)[C@@H](O[C@@H]3CO)Oc4ccc5C(C)=CC(=O)Oc5c4

InChI

1S/C34H47N3O18/c1-12-7-22(44)50-18-8-16(5-6-17(12)18)49-32-24(36-14(3)42)28(47)30(20(10-39)52-32)55-34-25(37-15(4)43)29(48)31(21(11-40)53-34)54-33-23(35-13(2)41)27(46)26(45)19(9-38)51-33/h5-8,19-21,23-34,38-40,45-48H,9-11H2,1-4H3,(H,35,41)(H,36,42)(H,37,43)/t19-,20-,21-,23-,24-,25-,26-,27-,28-,29-,30-,31-,32-,33+,34+/m1/s1

InChI key

BNYGKUQXGBVTRE-JFWBNMEYSA-N

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antibody form

purified immunoglobulin

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

mouse

biological source

-

biological source

mouse

clone

PSR-45, monoclonal

clone

PTR-8, monoclonal

clone

pT-154, monoclonal

clone

PT-66, monoclonal

technique(s)

dot blot: suitable, microarray: suitable, indirect ELISA: 0.3-0.6 μg/mL using phosphoserine conjugated to BSA, western blot: 2.5-5.0 μg/mL using total rat brain extract.

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1 μg/mL, microarray: suitable, western blot: 5-10 μg/mL using A431 cell extracts

technique(s)

direct ELISA: suitable, immunohistochemistry: suitable, microarray: suitable, western blot: 2-4 μg/mL using total extract of A431 cells stimulated by EGF

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: 0.5-1.0 μg/mL using phosphotyrosine conjugated to BSA, radioimmunoassay: suitable, western blot: 0.25-0.5 μg/mL using total cell extract of human platelets

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

Application

4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside has been used as a fluorogenic substrate to determine chitinase and chitotriosidase enzyme activity.

Substrates

Fluorogenic substrate for endochitinases, peptidoglycan muramidase, and lysozyme.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Gavriella Mavrikiou et al.
Clinical biochemistry, 49(12), 885-889 (2016-05-01)
The purpose of this study was to determine the normal range of chitotriosidase activity in the Cypriot population and the frequency of the 24bp duplication polymorphism. Furthermore, we compared the allele frequency of this polymorphism in two locations with different
Gerald Hochwimmer et al.
BMC microbiology, 9, 184-184 (2009-09-02)
The oomycete Aphanomyces astaci is regarded as the causative agent of crayfish plague and represents an evident hazard for European crayfish species. Native crayfish populations infected with this pathogen suffer up to 100% mortality. The existence of multiple transmission paths
Unnur Dilja Teitsdottir et al.
Sleep & breathing = Schlaf & Atmung, 22(4), 1101-1109 (2018-10-13)
The inflammatory markers chitinase-3-like protein 1 (CHI3L1) and chitotriosidase (CHIT1) have both been associated with cardiovascular complications. The aim of this preliminary observational study was to assess the roles and interaction of obstructive sleep apnea (OSA) severity and body mass
Michèle Reindl et al.
Frontiers in microbiology, 11, 1529-1529 (2020-08-01)
Subcellular targeting of proteins is essential to orchestrate cytokinesis in eukaryotic cells. During cell division of Ustilago maydis, for example, chitinases must be specifically targeted to the fragmentation zone at the site of cell division to degrade remnant chitin and
Michael B Howard et al.
Journal of bacteriology, 185(11), 3352-3360 (2003-05-20)
The marine bacterium Microbulbifer degradans strain 2-40 produces at least 10 enzyme systems for degrading insoluble complex polysaccharides (ICP). The draft sequence of the 2-40 genome allowed a genome-wide analysis of the chitinolytic system of strain 2-40. The chitinolytic system

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