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Safety Information

I4131

Sigma-Aldrich

IgG from rat serum

reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powder

Synonym(s):

Rat IgG

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About This Item

MDL number:
MFCD00164131
UNSPSC Code:
12352203
NACRES:
NA.46

CN¥5,713.28

Available to ship onApril 27, 2025Details

Request a Bulk Order

conjugate

unconjugated

Quality Level

300

grade

reagent grade

Assay

≥95% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

storage temp.

2-8°C

target post-translational modification

unmodified

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Show Differences

1 of 4

This Item
RAB0246RAB0251RAB0260
Mouse IL-10 ELISA Kit for serum, plasma and cell culture supernatant

RAB0245

Mouse IL-10 ELISA Kit

Rat IL-10 ELISA Kit for serum, plasma and cell culture supernatant

RAB0246

Rat IL-10 ELISA Kit

Mouse IL-11 ELISA Kit for serum, plasma and cell culture supernatant

RAB0251

Mouse IL-11 ELISA Kit

Mouse IL-15 ELISA Kit for serum, plasma and cell culture supernatant

RAB0260

Mouse IL-15 ELISA Kit

Gene Information

mouse ... Il10(16153)

Gene Information

rat ... Il10(25325)

Gene Information

mouse ... Il11(16156)

Gene Information

mouse ... Il15(16168)

species reactivity

mouse

species reactivity

rat

species reactivity

mouse

species reactivity

mouse

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type cell culture supernatant(s)
sample type serum
sample type plasma

input

sample type serum
sample type cell culture supernatant(s)
sample type plasma

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 45 pg/mL
standard curve range: 78.13-5000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 10 pg/mL
standard curve range: 8.23-6000 pg/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 1000 pg/mL
standard curve range: 1.85-450 ng/mL

assay range

inter-assay cv: <12%
intra-assay cv: <10%
sensitivity: 500 pg/mL
standard curve range: 0.33-80 ng/mL

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

technique(s)

ELISA: suitable, capture ELISA: suitable

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

General description

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids, Immunoglobins have two heavy (H) and two light (L) chains, linked by disulphide linkages. Each light chain comprises one variable N-terminal region and a constant C-terminal region. Heavy chain has one variable N-terminal region and three or four constant (CH1-CH4) C-terminal region.[1] The four classes of IgG can be IgG1, IgG2, IgG3, and IgG4.[2] IgG antibody provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Rat IgG is purified from pooled normal rat serum by fractionation and ion-exchange chromatography.

Application

IgG from rat serum has been used:
  • in immunolabeling[3]
  • in flow cytometry[4]
  • in enzyme linked immunosorbent assay[5]
  • as a control in neutrophil depletion assay[6]
Purified rat IgG may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. Rat IgG was used as isotype control in flow cytometry[7][8] and injected into mice to induce tolerance against rat IgG.[9]

Biochem/physiol Actions

IgG antibody have enormous therapeutic potential and its fragment crystallizable (Fc) region contributes for the development of therapeutic antibody.[10]

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

动植物源性产品

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    0000377031
    0000340200
    0000340196

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    Find documentation for the products that you have recently purchased in the Document Library.

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    Transcription and methylation analyses of preleukemic promyelocytes indicate a dual role for PML/RARA in leukemia initiation
    Gaillard C, et al.
    Haematologica, 100(8), 1064-1075 (2015)
    Neutrophils instruct homeostatic and pathological states in naive tissues
    Casanova-Acebes M, et al.
    The Journal of Experimental Medicine, 215(11), 2778-2795 (2018)
    Anuj Sehgal et al.
    Nature communications, 9(1), 1272-1272 (2018-03-30)
    Colony-stimulating factor 1 (CSF1) controls the growth and differentiation of macrophages.CSF1R signaling has been implicated in the maintenance of the intestinal stem cell niche and differentiation of Paneth cells, but evidence of expression of CSF1R within the crypt is equivocal.
    A Gugliucci et al.
    Life sciences, 62(23), 2141-2150 (1998-06-17)
    As the glycation/glycoxidation hypothesis for the genesis of diabetic complications is achieving widespread acceptance, much attention is being paid to the role of low molecular weight advanced glycation (AGE) adducts, as second generation glycating agents. We set out a study
    Comparative study of two protocols for quantitative image-analysis of serotonin transporter clustering in lymphocytes, a putative biomarker of therapeutic efficacy in major depression
    Romay-Tallon R, et al.
    Biomarker research, 5(1), 27-27 (2017)
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