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Safety Information

I3786

Sigma-Aldrich

Protease Inhibitor Cocktail

Animal-Free, DMSO solution, for the inhibition of serine, cysteine, aspartic proteases and aminopeptidases, for use with mammalian cell and tissue extract, DMSO solution

Synonym(s):

Protease inhibitor

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1 ML
CN¥435.52

CN¥435.52

Estimated to ship onJune 24, 2025Details

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1 ML
CN¥435.52

About This Item

UNSPSC Code:
12352200
NACRES:
NA.77
form:
DMSO solution
solubility:
water: soluble
storage temp.:
−20°C

CN¥435.52

Estimated to ship onJune 24, 2025Details

Product Name

Protease Inhibitor Cocktail, Animal Component Free, for use with mammalian cell and tissue extract, DMSO solution

Quality Level

200

form

DMSO solution

solubility

water: soluble

storage temp.

−20°C

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application(s)

petroleum

application(s)

environmental
petroleum

application(s)

environmental
petroleum

application(s)

environmental

grade

certified reference material, TraceCERT®

grade

certified reference material, TraceCERT®

grade

certified reference material

grade

certified reference material, TraceCERT®

product line

TraceCERT®

product line

TraceCERT®

product line

-

product line

TraceCERT®

CofA

current certificate can be downloaded

CofA

current certificate can be downloaded

CofA

current certificate can be downloaded

CofA

current certificate can be downloaded

packaging

ampule of 1 mL

packaging

ampule of 1 mL

packaging

ampule of 1 mL

packaging

ampule of 1 mL

General description

Components of this cocktail are of non-animal origin and no animal products were used in the component production process. All vessels and instruments used for the cocktail production are dedicated for animal component free production and have never encountered an animal product. Aprotinin used in this cocktail is a recombinant bovine protein expressed in plants (Nicotiana).
This protease inhibitor cocktail has been optimized and tested for mammalian cell and tissue extracts.

Specificity

Inhibits serine, cysteine, aspartic proteases, and aminopeptidases.

Application

Animal component free (ACF). Optimized and tested for general use. Can also be used for purification of histidine-tagged proteins, as chelating agents have been omitted.

Components

AEBSF
Aprotinin
Bestatin
E-64
Leupeptin
Pepstatin A

Physical form

The components in this ACF protease inhibitor cocktail do not originate from animal sources and can thus be used in the production of animal-free proteins.
This ACF cocktail is supplied as a ready-to-use solution in DMSO. One ml of the cocktail solution is recommended for the inhibition of endogenous enzymes found in 100 mL of lysate from 20 g (wet weight) of bovine liver, or 10 mL of cell lysate obtained from CHO cells at a cell density of 108 cells per mL.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Regulatory Information

常规特殊物品

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000231061
    0000231059
    0000231059
    0000231061
    0000198871

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