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P0044

Sigma-Aldrich

Phosphatase Inhibitor Cocktail 3

DMSO solution, for the inhibition of protein phosphatase 2A, alkaline phosphatases, protein phosphatases 1 and 2A, DMSO solution

Synonym(s):

Phosphatase inhibitor

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About This Item

EC Number:
200-664-3
UNSPSC Code:
12352200
NACRES:
NA.77
form:
DMSO solution
solubility:
water: soluble
storage temp.:
2-8°C
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Product Name

Phosphatase Inhibitor Cocktail 3, DMSO solution

form

DMSO solution

Quality Level

200

solubility

water: soluble

shipped in

wet ice

storage temp.

2-8°C

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manufacturer/tradename

Ultracel®

manufacturer/tradename

Ultracel®

manufacturer/tradename

Ultracel®

manufacturer/tradename

Ultracel®

material

regenerated cellulose membrane

material

regenerated cellulose membrane

material

regenerated cellulose membrane

material

regenerated cellulose membrane

technique(s)

protein clean-up: suitable (desalting)

technique(s)

protein clean-up: suitable (desalting), protein purification: suitable (concentration)

technique(s)

protein clean-up: suitable (desalting), protein purification: suitable (concentration)

technique(s)

protein clean-up: suitable (desalting), protein purification: suitable (concentration)

filter diam.

44.5 mm

filter diam.

25 mm

filter diam.

63.5 mm

filter diam.

76 mm

filtration area

13.4 cm2

filtration area

4.1 cm2

filtration area

28.7 cm2

filtration area

41.8 cm2

Application

The phosphatase inhibitor cocktail improves the yields of intact proteins by adding inhibitors to enzymes that modify proteins present in cell extracts. This product has been tested on extract from animal tissues including human placenta and bovine liver, and on extracts of A431, CHO, and U937 cells. It has been optimized and tested for L-isozymes of alkaline phosphatase as measured with pNPP and for serine-threonine protein phosphatases.

Biochem/physiol Actions

This mixture contains individual components with specific inhibitory properties. Cantharidin inhibits protein phosphatase 2A. (-)-p-Bromolevamisole oxalate inhibits L-isoforms of alkaline phosphatases. Calyculin A inhibits protein phosphatases 1 and 2A.

Caution

As supplied, this product is stable for four years at the recommended 2-8°C storage temperature.

Preparation Note

This cocktail is supplied as a clear solution in dimethyl sulfoxide. It is recommended to dilute the cocktail at 1 mL of cocktail per 500 mg of protein extracted from tissue or cells. Often a final concentration of 1% (v/v) is used, which will inhibit phosphatase activities found in the 27,000 x g supernatant from human placenta and bovine liver, and in the 100,000 x g supernatant from A431, CHO, and U937 extracts.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

188.6 °F

Flash Point(C)

87 °C

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    Lot/Batch Number
    0000440143
    0000440143
    0000234116
    0000234116
    0000398626

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    Phosphatase Inhibitor Cocktail

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