GE17-5143-02
HiTrap® Benzamidine Fast Flow
Cytiva 17-5143-02, pack of 2 × 1 mL
Recommended Products
ligand
p-aminobenzamidine
shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 2 × 1 mL
manufacturer/tradename
Cytiva 17-5143-02
storage condition
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
parameter
42 psi
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 4% agarose
average diameter
90 μm
cleaning
1-9
working range
2-8
storage temp.
2-8°C
Related Categories
General description
Application
The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.
Features and Benefits
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
- HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- High binding capacity.
- The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA® design or FPLC™ System.
Analysis Note
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Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Articles
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Protocols
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
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