GE17-5144-01
HiTrap® Benzamidine Fast Flow
Cytiva 17-5144-01, pack of 1 × 5 mL
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CN¥846.49
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About This Item
CN¥846.49
Recommended Products
ligand
p-aminobenzamidine
packaging
pack of 1 × 5 mL
manufacturer/tradename
Cytiva 17-5144-01
storage condition
(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
highly cross-linked 4% agarose
average diameter
90 μm
Related Categories
1 of 4
This Item | P6611 | H8162 | P9740 |
|---|---|---|---|
| capacity 15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein) | capacity >15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein) | capacity >15 mg/mL, agarose binding capacity (protein)(with an approx. 30 kDa protein) | capacity ≥2 μmol/mL, gel (phosphopeptide)(following a 30 minute incubation at 25°C) |
| technique(s) affinity chromatography: suitable | technique(s) protein purification: suitable | technique(s) protein purification: suitable | technique(s) - |
| form suspension | form (1:1 suspension in a 20% ethanol solution) | form (1:1 suspension in a 30% ethanol solution) | form suspension |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Application
The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.
Features and Benefits
- Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
- Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
- HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
- High binding capacity.
- The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA® design or FPLC™ System.
Storage and Stability
Analysis Note
Other Notes
Legal Information
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Regulatory Information
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Articles
This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from Cytiva.
This page shows troubleshooting strategies for cleavage methods using Cytiva products.
This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification of GST-tagged proteins in batch.
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Protocols
Protect fusion proteins from proteolytic degradation with enzyme removal pre- and post-cleavage using PreScission Protease, thrombin, or Factor Xa.
This page how to remove GST tags by enzymatic cleavage using Cytiva products.
Affinity chromatography tags like GST fusion proteins facilitate separations in various purification products from Cytiva.
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