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T4648

Sigma-Aldrich

Thrombin from bovine plasma

lyophilized powder, 40-300 NIH units/mg protein (biuret)

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Synonym(s):
Factor IIa
CAS Number:
9002-04-4
Enzyme Commission number:
3.4.21.5 (BRENDA, IUBMB)
EC Number:
232-648-7
MDL number:
MFCD00166053
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bovine plasma

Quality Level

300

form

lyophilized powder

specific activity

40-300 NIH units/mg protein (biuret)

mol wt

heavy chain ~33 kDa
light chain ~5 kDa

composition

Protein, 40-60%

UniProt accession no.

P00735

shipped in

wet ice

storage temp.

−20°C

Gene Information

cow ... F2(280685)

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General description

Thrombin is the final coagulation protease in regard to hemostasis, promoting both procoagulant and anticoagulant effects.

Application

Thrombin is used for site specific cleavage of recombinant fusion proteins containing an accessible thrombin recognition site for removal of affinity tags. Thrombin has been used in a study to assess persistent hypocoagulability in patients with septic shock.

Biochem/physiol Actions

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

Unit Definition

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

Physical form

Lyophilized powder containing sodium chloride and Tris-HCl, pH 7.0

Preparation Note

Activated with bovine brain thromboplastin.

Analysis Note

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.
The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

Other Notes

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H315,H319,H334,H335

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物源性产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the solubility of Product T4648, Thrombin from bovine plasma?

    Thrombin from bovine plasma is soluble in water at 10 mg/mL.

  6. What is the solution stability of Product T4648, Thrombin from bovine plasma?

    When stock solutions of thrombin from bovine plasma are prepared at a concentration of 100 units/mL in 0.1% (w/v) BSA, they are stable for one week at 0-5°C.  Since thrombin solutions adsorb to glass, it is recommended to aliquot the solutions in plastic tubes and store at -20°C for long term storage.

  7. What is the cross reactivity of Thrombin?

    Thrombin from any vertebrate species will clot the fibrinogen of virtually any other species.  Fibrinogen from any mammalian source will cross-react with thrombin from any mammalian source, but it is not well known how thrombin and fibrinogen from different animals differ in their sequence.  When any mammalian thrombin is injected into a different mammal, clotting will occur.

  8. What is the isoelectric point of Product T4648, Thrombin from bovine plasma?

    The isoelectric point of thrombin from bovine plasma is 7.05.

  9. How is the activity of Product T4648, Thrombin from bovine plasma, determined?

    The thrombin from bovine plasma activity is determined with a clotting assay (conversion of fibrinogen to fibrin) which measures alpha-thrombin and the amount of beta- and gamma-thrombin present is not measured. The assay of the clotting activity of thrombin from bovine plasma is performed at pH 7, utilizing a NIH thrombin reference standard.

  10. For thrombin, how do NIH units compare to International Units (IU)?

    One NIH unit of thrombin is equivalent to approximately 1.8 International Unit (IU) of thrombin.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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