GE17-1408-01
HiTrap® Desalting Columns
Cytiva 17-1408-01, pack of 5 × 5 mL
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shelf life
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
packaging
pack of 5 × 5 mL
manufacturer/tradename
Cytiva 17-1408-01
storage condition
dry media
parameter
42 psi
bed size
16 mm × 25 mm
bed volume
5 mL
column I.D.
16 mm
matrix
cross-linked dextran
particle size
>20 μm (dry)
cleaning
2-13
working range
2-13
suitability
suitable for bioprocess medium
Related Categories
General description
HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.
Application
Features and Benefits
- Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
- Replaces dialysis as the method of choice for desalting.
- Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.
Storage and Stability
Analysis Note
Other Notes
Legal Information
Signal Word
Warning
Hazard Statements
Precautionary Statements
Certificates of Analysis (COA)
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Articles
This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap™ FF columns and ÄKTAprime from Cytiva.
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This page shows how to perform a purification of His-tagged membrane proteins.
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Protocols
This page shows how to condition membrane proteins for further analysis with products from Cytiva.
Rules and good practice in sample preparation for Western blot sample preparation from cell culture and tissue samples.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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