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Safety Information

CS0410

Sigma-Aldrich

Glutathione-S-Transferase (GST) Assay Kit

sufficient for 500 multiwell tests

Synonym(s):

GST Enzyme Activity Kit

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About This Item

EC Number:
202-551-4
UNSPSC Code:
12161503
NACRES:
NA.84

This item is not able to be purchased as a guest user. For further information, please contact us at (86 21)8008193336,(86 21) 4006203333or

ordercn@merckgroup.com
.

usage

sufficient for 500 multiwell tests

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... GSTA4(2941) , GSTM1(2944)

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This Item
CS0260CS1020CGP1
Glutathione-S-Transferase (GST) Assay Kit sufficient for 500 multiwell tests

CS0410

Glutathione-S-Transferase (GST) Assay Kit

Glutathione Assay Kit sufficient for 700 assays

CS0260

Glutathione Assay Kit

Glutathione Assay Kit, Fluorimetric sufficient for 200 multiwell tests

CS1020

Glutathione Assay Kit, Fluorimetric

Glutathione Peroxidase Cellular Activity Assay Kit Sufficient for 100 colorimetric tests

CGP1

Glutathione Peroxidase Cellular Activity Assay Kit

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

usage

sufficient for 500 multiwell tests

usage

sufficient for 700 assays

usage

sufficient for 200 multiwell tests

usage

sufficient for 100 tests

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The Glutathione S-Transferase (GST) Assay Kit is designed to measure total GSTactivity. It can be used to measure GST activity in cell and bacterial lysates, tissue homogenates, and in plasma
and erythrocyte lysates

Application

The Glutathione S-Transferase Assay kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB), which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.

Biochem/physiol Actions

Glutathione S-transferases (GSTs) are a group of enzymes important in the detoxification of many xenobiotics in mammals. The enzymes protect cells against toxicants by conjugating the thiol group of the glutathione to electrophilic xenobiotics, and thereby defend cells against the mutagenic, carcinogenic, and toxic effects of the compounds. GST activity is present in plants, insects, yeast, bacteria, and most mammalian tissues especially in the liver, which plays a key role in detoxification.

Kit Components Only

Product No.
Description
  • Dulbecco's Phosphate Buffered Saline 100 mL
  • L-Glutathione Reduced 1 g
  • GST (Control) .1 mL
  • Sample Buffer 5 mL
  • Substrate (CDNB) 1.2 mL

Signal Word

Danger

Hazard Classifications

Acute Tox. 2 Dermal - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

危险化学品
含少量动物源组分生物产品
常规特殊物品

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0000423654
0000212520
0000212520
0000126464

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T Lin et al.
Toxicology, 235(1-2), 1-10 (2007-04-10)
Glutathione (GSH) is one of the most important antioxidants in mammalian cells. It also plays an important role in chemical detoxification. Some evidence showed that polycyclic aromatic hydrocarbons, such as benzo[a]pyrene (B[a]P [50-32-8]), could increase GSH content as a defense
S Melino et al.
Amino acids, 41(1), 103-112 (2010-03-10)
Natural organosulfur compounds (OSCs) have been shown to have chemopreventive effects and to suppress the proliferation of tumor cells in vitro through the induction of apoptosis. The biochemical mechanisms underlying the antitumorigenic and anti-proliferative effects of garlic-derived OSCs are not
Daniel Scotcher et al.
Drug metabolism and disposition: the biological fate of chemicals, 45(5), 556-568 (2017-03-09)
In vitro-in vivo extrapolation of drug metabolism data obtained in enriched preparations of subcellular fractions rely on robust estimates of physiologically relevant scaling factors for the prediction of clearance in vivo. The purpose of the current study was to measure
Maria Sehlstedt et al.
Particle and fibre toxicology, 7, 21-21 (2010-08-24)
Biomass combustion contributes to the production of ambient particulate matter (PM) in rural environments as well as urban settings, but relatively little is known about the health effects of these emissions. The aim of this study was therefore to characterize
Viviana Maresca et al.
Ecotoxicology and environmental safety, 163, 665-673 (2018-08-12)
This study evaluated the biological effects of highly polluted freshwater environment (Regi Lagni channels, S Italy) on the aquatic moss Leptodictyum riparium, exposed in bags at three sites representative of different environmental conditions and characterized by different heavy metal burdens.
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