19160
SOD Assay Kit
sufficient for 500 tests
Synonym(s):
Superoxide dismutase Assay Kit
About This Item
Recommended Products
usage
sufficient for 500 tests
Quality Level
availability
not available in USA
technique(s)
UV/Vis spectroscopy: suitable
suitability
corresponds for UV test (WST reagent, WST formazan)
use tested
storage temp.
2-8°C
General description
Application
Biochem/physiol Actions
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is contained in Product 19160, SOD Assay Kit (Superoxide dismutase)?
This kit contains WST Solution (1x5mL), Enzyme Solution (1x100μL), Buffer Solution (1x100mL), Dilution Buffer (1x50mL), and a manual.
What equipment is required to use Product 19160, SOD Assay Kit (Superoxide dismutase)?
The equipment needed to use this kit is a plate reader (450 nm filter), a 96-well microplate, 10 μL & 100-200 μL pipettes, a multichannel pipette and an incubator. Superoxide dismutase (SOD) is also necessary for the preparation of an inhibition curve.
How do I prepare a WST working solution for Product 19160, SOD Assay Kit (Superoxide dismutase)?
Dilute 1mL of WST Solution with 19mL of Buffer Solution.
When using Product 19160, SOD Assay Kit (Superoxide dismutase), how do I prepare an Enzyme working solution?
Centrifuge the Enzyme Solution tube for five seconds. Mix by pipeting, and dilute 15μL of Enzyme Solution with 2.5mL of Dilution Buffer.
When using Product 19160, SOD Assay Kit, how do I prepare a Superoxide Dismutase (SOD) working solution?
Dilute SOD with Dilution Buffer to prepare SOD standard Solutions if necessary for assay monitoring as follows: 200 U/mL, 100 U/mL, 50 U/mL, 20 U/mL, 10 U/mL, 5 U/mL, 1 U/mL, 0.1 U/mL, 0.05 U/mL, 0.01 U/mL, and 0.001 U/mL.
Are there any literature references for Product 19160, SOD Assay Kit (Superoxide dismutase)?
1. J. M. McCord and I. Fridovich, J. Biol. Chem., 244, 6049 (1969). 2. S. Goldstein and G. Czapski, Free Rad. Res. Comms.,12-13(Part 1), 5-10 (1991). 3. R. H. Burdon, V. Gill, and C. Rice-Evans, Free Rad. Res. Comms., 18, 369 (1993). 4. M. W. Sutherland and B. Learmonth, Free Rad. Res., 27, 283 (1997). 5. H. Ukeda, A. K. Sarker, D. Kawana and M. Sawamura, Anal. Sci., 15, 353 (1999). 6. H. Ukeda, D. Kawana, S. Maeda and M. Sawamura, Biosci. Biotechnol. Biochem., 63, 485 (1999). 7. A. V. Peskin, et al., Clin. Chim. Acta., 293, 157 (2000).
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