CORET
PCR Core kit with Taq DNA polymerase
PCR components in separate tubes to allow for optimization
Synonym(s):
PCR kit, Taq DNA Polymerase kit
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About This Item
UNSPSC Code:
41106300
eCl@ss:
32161000
NACRES:
NA.55
Quality Level
form
liquid
usage
sufficient for 250 reactions
feature
dNTPs included
hotstart: no
technique(s)
PCR: suitable
color
colorless
input
purified DNA
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. This enzyme is in a recombinant form, expressed in E. coli. Taq DNA polymerase enzyme can withstand high temperatures up to 95 °C without significant loss of activity. Taq DNA Polymerase is a magnesium ion-dependent enzyme. Taq DNA Polymerase possesses 5′→3′ DNA polymerase activity and 5′→3′ exonuclease activity.
Application
CORET PCR kit has been used for the amplification of genomic DNA during the study of genetic diversity in various species of Manilkara zapota.
PCR Core kit with Taq DNA polymerase is suitable for the amplification of genomic DNA, cDNA, or cloned DNA templates. Each component has been tested in the polymerase chain reaction. Optimal concentrations of Taq polymerase, template DNA, primers, and MgCl2 will depend on the specifics of the system. It may be necessary to optimize each component separately, especially the Taq and Mg2+ concentrations and the cycling parameters.
Quantity
Kit contains enough reagents for 250 units of Taq DNA polymerase.
Other Notes
The following reagents are required but not provided in the kit:
- Mineral Oil, M 8662 (optional)
- Thermocycler
- Primers
- DNA to be amplified
- Chloroform, C 7559 (optional)
Legal Information
No license is conveyed with the purchase of this product under any of US Patents Nos. 5,804,375, 5,994,056, 6,171,785, 6,214,979, 5,538,848, 5,723,591, 5,876,930, 6,030,787, and 6,258,569, and corresponding patents outside the United States, or any other patents or patent applications, relating to the 5′ Nuclease and dsDNA-Binding Dye Processes. For further information contact the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Kit Components Also Available Separately
Product No.
Description
SDS
- P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included 1 mL/vialSDS
- P231710X PCR Buffer without MgCl2, Optimized for routine PCR without MgCl2 1 mL/vialSDS
- M8787Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 1 mL/vialSDS
- W1754Water, PCR Reagent 3 vial(s)SDS
- D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent .25 mLSDS
Storage Class Code
12 - Non Combustible Liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
常规特殊物品
含少量动物源组分生物产品
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Genetic diversity and structure of the tree Manilkara zapota in a naturally fragmented tropical forest
Martiinez N, et al.
Journal of tropical medicine, 33(4), 285-294 (2017)
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 14-14 (1989)
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