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D1806

Sigma-Aldrich

Taq DNA Polymerase from Thermus aquaticus

with 10× PCR reaction buffer containing MgCl2

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Synonym(s):
MgCl2 taq polymerase, Taq polymerase
CAS Number:
9012-90-2
Enzyme Commission number:
2.7.7.7 (BRENDA, IUBMB)
MDL number:
MFCD00166026
UNSPSC Code:
12352204
NACRES:
NA.55

biological source

enzyme from bacterial (Thermus Aquaticus)

Quality Level

200

recombinant

expressed in E. coli

form

liquid

usage

sufficient for 10000 reactions
 sufficient for 3000 reactions
 sufficient for 500 reactions

mol wt

94 kDa

feature

dNTPs included: no
hotstart: no

concentration

5 units/μL

technique(s)

PCR: suitable

color

colorless

input

purified DNA

suitability

suitable for PCR and automated sequencing reactions

application(s)

agriculture

shipped in

wet ice

storage temp.

−20°C

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General description

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.

Application

Taq DNA Polymerase from Thermus aquaticus has been used:
  • in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assay
  • in conventional reverse transcriptase (RT)-PCR
  • in simple sequence repeats (SSR) genotyping
  • as a component of PCR mix for amplification of genomic and mitochondrial DNA
  • in direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

  • Low per unit cost of Taq

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl2
Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

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Regulatory Information

常规特殊物品
含少量动物源组分生物产品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Protocols

dNTP Hot Start PCR Protocol

Hot Start dNTPs block DNA polymerase until heat activation, enhancing PCR specificity.

Standard PCR Protocol

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

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