Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

Merck
CN
All Photos(2)

Key Documents

Safety Information

B7931

Sigma-Aldrich

Anti-BNIP3 antibody, Mouse monoclonal

clone ANa40, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-BNIP3 antibody produced in mouse

Sign Into View Organizational & Contract Pricing

Select a Size

0.2 ML
CN¥5,432.63

CN¥5,432.63

Please contact Customer Service for Availability
Request a Bulk Order
All Photos(2)

Select a Size

Change View
0.2 ML
CN¥5,432.63

About This Item

MDL number:
MFCD03457547
UNSPSC Code:
12352203
NACRES:
NA.41

CN¥5,432.63

Please contact Customer Service for Availability
Request a Bulk Order

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

ANa40, monoclonal

form

buffered aqueous solution

mol wt

antigen 21.5 kDa

species reactivity

human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 2-4 μg/mL using whole extract of transfected 293T (human embryonal kidney) cells expressing human BNIP3.

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB1408551SAB5700815V9264
Anti-BNIP3 antibody, Mouse monoclonal clone ANa40, purified from hybridoma cell culture

B7931

Anti-BNIP3 antibody, Mouse monoclonal

Anti-BNIP3 antibody produced in rabbit purified immunoglobulin, buffered aqueous solution

SAB1408551

Anti-BNIP3 antibody produced in rabbit

Anti-BNIP3 antibody produced in rabbit

SAB5700815

Anti-BNIP3 antibody produced in rabbit

Anti-Vinculin antibody, Mouse monoclonal clone hVIN-1, purified from hybridoma cell culture

V9264

Anti-Vinculin antibody, Mouse monoclonal

biological source

mouse

biological source

rabbit

biological source

rabbit

biological source

mouse

Gene Information

human ... BNIP3(664)

Gene Information

human ... BNIP3(664)

Gene Information

human ... BNIP3(664)

Gene Information

human ... VCL(7414)
mouse ... Vcl(22330)
rat ... Vcl(305679)

species reactivity

human

species reactivity

human

species reactivity

mouse, human, rat

species reactivity

frog, chicken, mouse, canine, human, bovine, rat, turkey

clone

ANa40, monoclonal

clone

polyclonal

clone

polyclonal

clone

hVIN-1, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

General description

Monoclonal Anti-BNIP3 (mouse IgG2b isotype) is derived from the ANa40 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with a recombinant human BNIP3. B-cell lymphoma 2 (BCL2) Interacting Protein 3 (BNIP3) is a pro-apoptotic protein localized in mitochondria and belongs to Bcl-2 family. Its Bcl-2 homology 3 (BH3) domain is less conserved in mammalian cells and yeast.

Specificity

Rabbit monoclonal clone ANa40 anti-BNIP3 recognizes human BNIP3. The epitope recognized by the antibody resides within amino acids 112-124 of human BNIP3 molecule.

Immunogen

recombinant human BNIP3 (amino acids 1-163, BNIP3ΔTM2).

Application

Anti-BNIP3 antibody, Mouse monoclonal has been used in
  • western blotting
  • immunoblotting
  • immunocytochemistry
  • immunoprecipitation enzyme linked immunosorbent assay (ELISA)
  • immunofluorescence analysis

Rabbit monoclonal clone ANa40 anti-BNIP3 is used to tag BNIP3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as ELISA, immunoblotting, and immunoprecipitation. It is used as a probe to determine the presence and roles of BNIP3 in the regulation of mitochondrial and non-mitochondrial mediated apoptosis.
The product is useful in ELISA, immunoblotting (21.5 kDa, apparent molecular mass in SDS-PAGE is approximately 30 and 60 kDa, for monomer and dimer, respectively), immunocytochemistry (4% formaldehyde), and immunoprecipitation.

Biochem/physiol Actions

B-cell lymphoma 2 (BCL2) Interacting Protein 3 (BNIP3) functions without a typical Bcl-2 homology 3 (BH3) domain. It interacts withcell death abnormality gene 9 (CED-9). BNIP3 interaction with B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma-extra-large (Bcl-XL) regulates apoptosis in mitochondria l and non-mitochondrial sites. Endogenous BNIP3 is loosely associated with mitochondrial membrane in normal tissue but fully integrates into the mitochondrial outer membrane with the N-terminus in the cytoplasm and the C-terminus in the membrane during induction of cell death. This is accompanied by rapid and profound mitochondrial dysfunction characterized by opening of the mitochondrial permeability transition (PT) pore, proton electrochemical gradient (Dym) suppression, and increased reactive oxygen species production.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品
  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000242784
    0000242784
    0000131880
    0000100855
    128M4890V

    Don't see the Right Version?

    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

    Search for a COA

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Yanxiang Shao et al.
    International journal of oncology, 54(1), 348-360 (2018-10-27)
    The majority of clear cell renal cell carcinomas (ccRCCs) are caused by an accumulation of hypoxia‑inducible factor (HIF) and the overexpression of downstream genes in response to the von Hippel‑Lindau (VHL) gene becoming inactivated. In the present study, our hypothesis
    Bnip3-mediated mitochondrial autophagy is independent of the mitochondrial permeability transition pore
    Quinsay MN, et al.
    Autophagy, 6(7), 855-862 (2010)
    Autophagic Signaling and Proteolytic Enzyme Activity in Cardiac and Skeletal Muscle of Spontaneously Hypertensive Rats following Chronic Aerobic Exercise
    McMillan EM, et al.
    PLoS ONE, 10(3), e0119382-e0119382 (2015)
    BNIP3 protects HepG2 cells against etoposide-induced cell death under hypoxia by an autophagy-independent pathway
    Cosse JP, et al.
    Biochemical Pharmacology, 80(8), 1160-1169 (2010)
    Bnip3 as a Dual Regulator of Mitochondrial Turnover and Cell Death in the Myocardium
    Gustafsson AA, et al.
    Pediatric Cardiology, 32(3), 267-267 (2011)
    View All Related Papers

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service