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T6793

Sigma-Aldrich

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse

clone 6-11B-1, ascites fluid

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Synonym(s):
Anti-ALS22, Anti-H2-ALPHA, Anti-TUBA1
MDL number:
MFCD00164512
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6-11B-1, monoclonal

contains

15 mM sodium azide

species reactivity

mouse, protista, invertebrates, hamster, plant, frog, rat, monkey, pig, bovine, chicken, human

technique(s)

dot blot: suitable
electron microscopy: suitable
immunocytochemistry: suitable
indirect ELISA: suitable
radioimmunoassay: suitable
western blot: 1:2,000 using a bovine or rat brain preparation

isotype

IgG2b

UniProt accession no.

P68366

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBA4A(7277)
mouse ... Tuba1a(22142)
rat ... Tuba1a(64158)

General description

α-tubulin, also called as tubulin α 4a (TUBA4A), is mapped to human chromosome 2q35. The gene codes for a member of the α-tubulin family containing 448 amino acids. TUBA4A belongs to the subfamily of tubulin, which is the major building block of microtubules. α-subunit of tubulin has molecular weight of 50,000.

Specificity

The antibody recognizes an epitope located on the α3 isoform of Chlamydomonas axonemal α-tubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very similar to the one detected by the antibody in Chlamydomonas is found in the majority of α-tubulins, but the corresponding region is markedly divergent in some α-tubulin isoforms from chicken, Drosophila and yeast. The antibody has been used to detect acetylated α-tubulins in several organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it is in the kangaroo rat epithelial-like cell line PtK2.

Immunogen

acetylated α-tubulin from the outer arm of sea urchin sperm axonemes.

Application

Monoclonal Anti-Tubulin, Acetylated antibody produced in mouse has been used in following studies:
  • immunofluorescence.
  • western blotting.
  • immunocytochemistry.
  • immunohistochemistry.

Biochem/physiol Actions

Acetylated tubulin is associated with axoneme assembly in a variety of organisms. Acetylated tubulin might play a crucial role in the differentiation of microtubule structure and function. Post translational modification of α-tubulin i.e. the reversible addition of an acetyl group to Lys40, by specific acetylase, results in acetylated tubulin.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Molecular architecture of the centriole proteome: the conserved WD40 domain protein POC1 is required for centriole duplication and length control.
Keller LC
Molecular Biology of the Cell, 20(4), 1150-1166 (2009)
Ryan R Roberts et al.
Development (Cambridge, England), 146(15) (2019-07-20)
Tendon and bone are attached by a transitional connective tissue that is morphologically graded from tendinous to osseous and develops from bipotent progenitors that co-express scleraxis (Scx) and Sox9 (Scx+/Sox9+). Scx+/Sox9+ progenitors have the potential to differentiate into either tenocytes
Georg Mayer et al.
Proceedings. Biological sciences, 276(1673), 3571-3579 (2009-07-31)
Despite the advent of modern molecular and computational methods, the phylogeny of the four major arthropod groups (Chelicerata, Myriapoda, Crustacea and Hexapoda, including the insects) remains enigmatic. One particular challenge is the position of myriapods as either the closest relatives
Acetylation: a regulatory modification to rival phosphorylation?
Kouzarides T
The Embo Journal, 19, 1176-1179 (2000)
Nanomechanical property maps of breast cancer cells as determined by multiharmonic atomic force microscopy reveal Syk-dependent changes in microtubule stability mediated by MAP1B.
Krisenko MO, et.al.
Biochemistry, 54, 60-68 (2015)
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Articles

Antibodies to Tubulins, including Centrosome-Associated ε-Tubulin

Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of α- and β-tubulin. In addition to α-and β-tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven.

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