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SAB4200761

Sigma-Aldrich

Anti-Vimentin antibody, Mouse monoclonal

enhanced validation

clone LN-6, purified from hybridoma cell culture

Synonym(s):

Anti-VIM

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

200

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

LN-6, monoclonal

form

buffered aqueous solution

mol wt

~58 kDa

species reactivity

rat, human, porcine, monkey, bovine, canine, mouse, rabbit, hamster

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

flow cytometry: suitable
immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast Hs68 cell line.
immunofluorescence: 1-2 μg/mL using human foreskin fibroblast Hs68 cell line.
immunohistochemistry: 5-10 μg/mL using Human breast carcinoma sections.
immunoprecipitation (IP): suitable

isotype

IgM

UniProt accession no.

P08670

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

bovine ... Vim(280955)
dog ... Vim(477991)
hamster ... Vim(100689099)
human ... VIM(7431)
mouse ... Vim(22352)
pig ... Vim(100522394)
rabbit ... Vim(100008924)
rat ... Vim(81818)
rhesus monkey ... Vim(705289)

Related Categories

General description

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the LN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse. Vimentin protein is localized in a wide range of cell types, including pancreatic precursor cells, sertoli cells, mesangial cells, trophoblastic giant cells, neuronal precursor cells, fibroblasts, macrophages, endothelial cells of lung blood vessels, renal tubular cells, neutrophils, leukocytes and renal stromal cells.
Vimentin is a specific type III intermediate filament (IF) protein. It is abundantly expressed in mesenchymal cells. The gene encoding vimentin is located on human chromosome 10p13.

Specificity

Anti-Vimentin antibody, Mouse monoclonal specifically recognizes a unique epitope of Vimentin protein which is not expressed in cells of hematopoetic derivation. The antibody is reactive with benign and malignant tumors of mesenchymal and neural derivation but does not react with normal and malignanat human lymphoid cells or derived malignancies. The antibody reacts with Vimentin from human, hamster, mouse, rat, canine, monkey, bovine, porcine and rabbit origin.

Immunogen

Human thymic nuclear extract

Application

Anti-Vimentin antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • flow cytometry
  • immunoprecipitation
  • immunohistochemistry

Biochem/physiol Actions

Vimentin helps to preserve the structure of cells and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions.
Vimentin protein plays a significant role in supporting and anchoring the cellular position of organelles such as the nucleus, endoplasmic reticulum, mitochondria, maintenance of cell shape, the endurance of mechanical stress of mesenchymal cells and motility. Overexpression of the vimentin gene is observed in various epithelial cancers, including prostate, gastrointestinal tumors, central nervous system (CNS) tumors, breast, malignant melanoma and lung cancer.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

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The gene encoding human vimentin is located on the short arm of chromosome 10.
Ferrari S E R G I O, et al.
American Journal of Human Genetics, 41(4), 616-616 (1987)
Vimentin modulates infectious internalisation of HPV16 pseudovirions.
Schafer G, et al.
Journal of Virology, 91(16), e00307-e00317 (2017)
Viscoelastic properties of vimentin compared with other filamentous biopolymer networks.
Janmey PA, et al.
The Journal of Cell Biology, 113(1), 155-160 (1991)
Arun Satelli et al.
Cellular and molecular life sciences : CMLS, 68(18), 3033-3046 (2011-06-04)
Vimentin, a major constituent of the intermediate filament family of proteins, is ubiquitously expressed in normal mesenchymal cells and is known to maintain cellular integrity and provide resistance against stress. Vimentin is overexpressed in various epithelial cancers, including prostate cancer
Vimentin is a potential prognostic factor for tongue squamous cell carcinoma among five epithelial?mesenchymal transition-related proteins.
Liu P F, et al.
PLoS ONE, 12(6), e0178581-e0178581 (2017)
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