A2547
Monoclonal Anti-Actin, α-Smooth Muscle
clone 1A4, ascites fluid
Synonym(s):
Alpha Smooth Muscle Actin Antibody - Monoclonal Anti-Actin, α-Smooth Muscle, Alpha Smooth Muscle Actin Antibody Sigma, Anti-Alpha Smooth Muscle Actin Antibody, SMA
About This Item
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
1A4, monoclonal
mol wt
antigen ~42 kDa
contains
15 mM sodium azide
species reactivity
human, mouse, rat, chicken, frog, canine, rabbit, guinea pig, goat, bovine, sheep, snake
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using smooth muscle cells
immunohistochemistry (frozen sections): suitable using smooth muscle cells
indirect immunofluorescence: 1:400 using blood vessels in sections of human appendix
western blot: suitable using smooth muscle cells
isotype
IgG2a
UniProt accession no.
application(s)
research pathology
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... ACTA2(59)
mouse ... Acta2(11475)
rat ... Acta2(81633)
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General description
Mouse monoclonal anti-actin, α-smooth muscle antibody binds to actin from human, mouse, rat, bovine, chicken, frog, goat, guinea pig, rabbit, dog, sheep, and snake.
Monoclonal Anti-α Smooth Muscle Actin (mouse IgG2a isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. The NH2 terminal synthetic decapeptide of α-smooth muscle actin coupled to keyhole limpet hemocyanin (KLH) was used as the immunogen. The isotype is determined using Sigma ImmunoTypeTM Kit (Sigma Stock No. ISO-1) and by a double diffusion immunoassay using Mouse Monoclonal Antibody Isotyping Reagents (Sigma Stock No. ISO-2).
Specificity
Immunogen
Application
Monoclonal Anti-Actin, α-Smooth Muscle antibody has been used in the detection of actin 2 in breast fibroblasts using immunofluorescence staining.
IHC analysis of x-gal stained muouse cardiac tissue was performed using the primary antibody, mouse monoclonal anti-smooth muscle actin to identify myofibroblasts.
Biochem/physiol Actions
Mutations in actin 2 is associated with dysfunction of smooth muscles in arteries and is implicated in coronary heart disease. Mutations in ACTA2 contributes to tear and enlargement of aorta in thoracic aortic aneurysms (TAAD).
Physical form
Storage and Stability
Other Notes
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Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
Choose from one of the most recent versions:
Certificates of Analysis (COA)
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the molecular weight of actin, Products A2066 and A2547?
Actin has a molecular weight of 42 kDa.
Why do I have 3 bands on my western blot when using Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse?
While actin should run as a 42 kDa band when detected by Western blotting, there are recent observations that cleavage products can be seen in cells undergoing apoptosis. Initial cleavage gives a band at 41 kDa, which may appear as a doublet on the blot. In addition, further cleavage results in a major band at 30 kDa and a small band at 14 kDa which have been shown to be cleavage products due to the action of interleukin 1 -converting enzyme. This may explain the detection of smaller bands when blotting with this antibody. This banding pattern may be detected with other actin antibodies as well. Kayalar, C., et al., Proc. Natl. Acad. Sci., USA, 93:2234-2238, (1996).
What is the antibody concentration of Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse?
This antibody is ascities fluid. The amount of protein present and the amount of IgG1 present is indicated on the Lot-specific Certificate of Analysis.
Is there a protocol for using Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody produced in mouse, in immunohistochemistry?
This antibody is used in our Immunohistochemistry Kit, Product No. IMMH2.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Can Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody detect beta actin?
No, Product A2547, Monoclonal Anti-Actin, α-Smooth Muscle antibody will not cross react with beta actin.
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