B3795
Brain-derived neurotrophic factor human
BDNF, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym(s):
Abrineurin, BDNF
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About This Item
biological source
human
Quality Level
recombinant
expressed in E. coli
Assay
≥95% (SDS-PAGE)
form
lyophilized powder
quality
endotoxin tested
mol wt
~27 kDa
packaging
pkg of 10 μg
pkg of 5 μg
storage condition
avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)
technique(s)
cell culture | mammalian: suitable
impurities
≤1.00 EU/μg Endotoxin level
UniProt accession no.
storage temp.
−20°C
Related Categories
General description
BDNF is a member of the neurotrophin family of growth factors that includes NGF, NT-3, and NT-4. All neurotrophins have six conserved cysteine residues and share a 55% sequence identity at the amino acid level. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.1 BDNF is expressed within peripheral ganglia and is not restricted to neuronal target fields, raising the possibility that BDNF has paracrine or even autocrine actions on neurons as well as non-neuronal cells.
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
The active form of BDNF recombinant human protein (27 kDa) is a dimer formed by two identical 119 amino acid subunits held together by strong hydrophobic interactions
Application
Brain-derived neurotrophic factor (BDNF) recombinant human protein has been used as a supplement in various types of culture media.
Biochem/physiol Actions
Brain-derived neurotrophic factor (BDNF) is a 13.6 kDa (or 27.2 kDa dimer) member of the neurotrophin family. BDNF has identical amino acid sequence in human, mouse, and pig with full cross-reactivities. BDNF is important in development and maintenance of neuronal populations within the central nervous system or cells directly associated with it. BDNF has been shown to enhance the survival and differentiation of several classes of neurons in vitro, including neural crest and placode-derived sensory neurons, dopaminergic neurons in the substantia nigra, basal forebrain cholinergic neurons, hippocampal neurons, and retinal ganglial cells.
Physical form
lyophilized from a sterile 0.2 μm filtered solution with BSA
Analysis Note
The biological activity is determined by its ability to stimulate the proliferation of human neuroblastoma cells, SH-SY5Y.
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
监管及禁止进口产品
Certificates of Analysis (COA)
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Lisa Oliver et al.
Stem cells and development, 21(15), 2779-2788 (2012-04-24)
Autophagy plays an important role in homeostasis, development, and disease, functioning both as a survival and cell death pathway. However, despite its importance in cell physiology, there is little information about the role of autophagy in stem cells and, in
Differentiation and histological analysis of embryonic stem cell-derived neural transplants in mice.
Y Benninger et al.
Brain pathology (Zurich, Switzerland), 10(3), 330-341 (2000-07-08)
We report here that neural transplantation of in vitro-differentiated embryonic stem (ES) cells provides a versatile strategy for gene transfer into the central nervous system. ES cells were subjected to an optimized in vitro differentiation protocol to obtain embryoid bodies.
Bo Tian et al.
Nature cell biology, 11(2), 211-218 (2009-01-20)
The phosphatidylinositol-3-kinase-like kinase ATM (ataxia-telangiectasia mutated) has a central role in coordinating DNA damage responses, including cell-cycle checkpoint control, DNA repair and apoptosis. Mutations of ATM cause a spectrum of defects ranging from neurodegeneration to cancer predisposition. However, the mechanism
Lisa Oliver et al.
PloS one, 6(5), e19820-e19820 (2011-05-19)
Adult mesenchymal stem cells (MSCs) can be maintained over extended periods of time before activation and differentiation. Little is known about the programs that sustain the survival of these cells. Undifferentiated adult human MSCs (hMSCs) did not undergo apoptosis in
W Sun et al.
Neuroscience, 164(4), 1854-1866 (2009-09-26)
Cochlear spiral ganglion neurons (SGN) provide the only pathway for transmitting sound evoked activity from the hair cells to the central auditory system. Neurotrophic factor 3 (NT-3) and brain derived neurotrophic factor (BDNF) released from hair cells and supporting cells
Protocols
Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.
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