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B2787

Sigma-Aldrich

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

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Synonym(s):
protein markers, protein molecular weight markers, protein standards
UNSPSC Code:
12352200
eCl@ss:
34058011
NACRES:
NA.25

form

lyophilized powder

Quality Level

200

usage

 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

mol wt

6,500-180,000 Da

packaging

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

storage temp.

−20°C

Related Categories

Application

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.

Components

This product contains:
  • α2-macroglobulin (equine serum)
  • β-galactosidase (E. coli)
  • phosphorylase b (rabbit muscle)
  • catalase (bovine liver)
  • alcohol dehydrogenase (yeast)
  • carbonic anhydrase (bovine erythrocyte)
  • trypsin inhibitor (soy bean)
  • lysozyme (chicken egg)
  • aprotinin (bovine lung)
Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

Other Notes

Contains nine biotinylated proteins (approx 0.1 mg total).

Reconstitution

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

常规特殊物品

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. The data sheets for Products SDS7B2 and B2787 do not provide a specific recommendation on how long the solutions of these molecular weight marker mixtures can be kept frozen at -20°C.  What information can you provide?

    Our R&D group recommends that after reconstitution, the solutions should be divided into aliquots and immediately frozen; avoid repeated freeze / thaw cycles. The aliquots should last at least a year at -20°C. Storage at -80°C would even be better, if it is convenient.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)

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