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Key Documents

69079

Sigma-Aldrich

Perfect Protein Markers, 10-225 kDa

Synonym(s):

protein markers, protein molecular weight markers, protein standards

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Select a Size

20 PKG
CN¥935.90
200 PKG
CN¥9,325.80

CN¥935.90


Estimated to ship onJune 20, 2025Details



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20 PKG
CN¥935.90
200 PKG
CN¥9,325.80

About This Item

UNSPSC Code:
41105337
NACRES:
NC.01

CN¥935.90


Estimated to ship onJune 20, 2025Details


form

liquid

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

shipped in

wet ice

storage temp.

−20°C

General description

The Perfect Protein<TMSYMBOL></TMSYMBOL> Markers are a novel set of recombinant proteins with definedsizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gelelectrophoresis, the Perfect Protein Markers enable highly accurate size determination ofunknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).

The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).

The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).

The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.

Features and Benefits

• Markers are supplied at the working dilution ingel loading buffer; concentration optimized forCoomassie blue staining

• Recombinant, unmodified markers producesharp, accurately sized bands

• Available in two size ranges:

15-150 kDa

10-225 kDa

Warning

Toxicity: Toxic (F)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


  • Certificates of Analysis (COA)

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    Rebeccah A Warmack et al.
    The Journal of biological chemistry, 294(32), 12203-12219 (2019-06-27)
    Transparency in the lens is accomplished by the dense packing and short-range order interactions of the crystallin proteins in fiber cells lacking organelles. These features are accompanied by a lack of protein turnover, leaving lens proteins susceptible to a number
    Csenge Filep et al.
    Analytical chemistry, 92(5), 4023-4028 (2020-02-06)
    The temperature-dependent migration of molecular weight protein size standards and several biotherapeutic proteins were studied in sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) in the interval from 15 to 60 °C using borate cross-linked dextran sieving matrix. Arrhenius plots were

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