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Key Documents

Safety Information

M3546

Sigma-Aldrich

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600)

Synonym(s):

protein markers, protein molecular weight markers, protein standards

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About This Item

UNSPSC Code:
41105337
NACRES:
NA.25

usage

 vial sufficient for 400 applications on large gels (16 × 18 cm)
 vial sufficient for 800 mini-gels (10 × 10 cm)

Quality Level

packaging

vial of 200 μL

storage temp.

−20°C

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This Item
GREL5Z742616GE80648470
manufacturer/tradename

G-BioSciences 786-947

manufacturer/tradename

Millipore

manufacturer/tradename

G-BioSciences 786-948

manufacturer/tradename

Cytiva 80-6484-70

packaging

pkg of 10 ea

packaging

pkg of 5 packs

packaging

pkg of 30 ea

packaging

pkg of 50 samples

feature

10 columns

feature

-

feature

30 columns

feature

wetted part: no

storage temp.

2-8°C

storage temp.

-

storage temp.

2-8°C

storage temp.

-

Application

Ultra-low Range Molecular Weight Marker (M.W. 1,060-26,600) has been used as a protein marker in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and western blot.[1][2][3]

Biochem/physiol Actions

The ultralow range marker is designed for use in the Tris-Tricine SDS-PAGE method when stained with Brilliant blue G (non-methanol based). Glutaraldehyde fixing is recommended with the ultralow marker in order to keep the two smallest proteins fixed in the gel. The ultralow range marker is supplied as a liquid, which must be diluted (1:20) with the supplied sample buffer prior to loading.

Physical form

Solution in 0.1 M Tris-HCl, pH approx. 8.5, with 4 mM EDTA, 3 mM sodium azide and 40% glycerol

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Endostatin enhances radioresponse in breast cancer cells via alteration of substance P levels.
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Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system.
Morassutti C, et al.
Febs Letters, 519, 141-146 (2002)
Christelle Richard et al.
Applied and environmental microbiology, 70(1), 248-254 (2004-01-09)
Polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized C-terminal part of divercin V41 (DvnCt) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and reactivity of the DvnCt-KLH-generated antibodies were evaluated by enzyme-linked
Raquel Soares Oliveira et al.
Toxins, 12(6) (2020-06-19)
The biological activity of Rhinella icterica parotoid secretion (RIPS) and some of its chromatographic fractions (RI18, RI19, RI23, and RI24) was evaluated in the current study. Mass spectrometry of these fractions indicated the presence of sarmentogenin, argentinogenin, (5β,12β)-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide, marinobufagin, bufogenin
Thiago Machado Pasin et al.
Applied biochemistry and biotechnology, 191(3), 1111-1126 (2020-01-22)
The use of non-potable water (such as seawater) is an attractive alternative for water intensive processes such as biomass pretreatment and saccharification steps in the production of biochemicals and biofuels. Identification and application of halotolerant enzymes compatible with high-salt conditions

Protocols

Separation of various acids and compounds by molecular weight: PAA, PEI, PIDADMACl, PAS, cationic dextran, chitosan

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