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Key Documents

B2025

Sigma-Aldrich

Brilliant Blue G - Colloidal Concentrate

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CN¥678.00

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.85

CN¥678.00

Please contact Customer Service for Availability

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solution

storage temp.

room temp

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This Item
B0770B8647B0149
Brilliant Blue G - Colloidal Concentrate

B2025

Brilliant Blue G - Colloidal Concentrate

Brilliant Blue G pure

B0770

Brilliant Blue G

Brilliant Blue R Concentrate suitable for SDS-PAGE, methanol solution

B8647

Brilliant Blue R Concentrate

Brilliant Blue R Dye content ~50 %, Technical grade

B0149

Brilliant Blue R

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE).[1] Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction.[2] It is effective in staining anterior capsule in cornea with relatively less toxic effects.[3]

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
  • protein fragments post in vitro pepsin digestion[4]
  • ovarian tumor fluid proteome prior to profiling[5][6]
  • the transcription factor (TFIID) complex from Saccharomyces cerevisiae[7]

Reconstitution

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

related product

Product No.
Description
Pricing

Pictograms

Corrosion
GHS05

Signal Word

Warning

Hazard Statements

H290,H315,H319

Hazard Classifications

Eye Irrit. 2 - Met. Corr. 1 - Skin Irrit. 2

Storage Class Code

8B - Non-combustible corrosive hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

  • Specification Sheet

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    SLCQ0016
    SLCJ6088
    SLCC7506
    SLBT3592
    SLBQ0154V

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    If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How would you recommend that I reconstitute Product B2025, Brilliant Blue G?

      One bottle of the product makes 1 liter of working solution.  The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.

    4. What is the solution stability of Product B2025, Brilliant Blue G?

      Once diluted the solution should be stable indefinitely.  It should be stored in the refrigerator and shaken well before each use.  The solution tends to precipitate on standing and heat accelerates precipitation.  Therefore, do not leave the bottle at room temperature overnight.

    5. Can you use Product B2025, Brilliant Blue G, with nitrocellulose?

      Use with nitrocellulose is not recommended, due to the methanol.

    6. How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?

      The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.

    7. What is the detection limit of Product B2025, Brilliant Blue G?

      The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins

    8. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    9. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    10. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Elena Barbieri et al.
    Journal of aging research, 2011, 845379-845379 (2011-06-02)
    This study describes mitochondrial behaviour during the C2C12 myoblast differentiation program and proposes a proteomic approach to mitochondria integrated with classical morphofunctional and biochemical analyses. Mitochondrial ultrastructure variations were determined by transmission electron microscopy; mitochondrial mass and membrane potential were
    Proteomic analysis of ovarian cancer tumor fluid is a rich source of potential biomarkers
    Poersch A, et al.
    Journal of Proteomics and Bioinformatics, 5(2), 2-2 (2014)
    Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis
    Candiano G, et al.
    Electrophoresis, 25(9), 1327-1333 (2004)
    V Neuhoff et al.
    Electrophoresis, 9(6), 255-262 (1988-06-01)
    An improved procedure for staining of proteins following separation in polyacrylamide gels is described which utilizes the colloidal properties of Coomassie Brilliant Blue G-250 and R-250. The new method is based on addition of 20% v/v methanol and higher concentrations
    Staining ability and biocompatibility of brilliant blue G: preclinical study of brilliant blue G as an adjunct for capsular staining
    Hisatomi T, et al.
    Archives of Ophthalmology, 124(4), 514-519 (2006)
    View All Related Papers

    Protocols

    Brilliant Blue G – Colloidal Concentrate Protocol

    Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service