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B8647

Sigma-Aldrich

Brilliant Blue R Concentrate

methanol solution

Synonym(s):

Brilliant Blue R, Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R

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About This Item

Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
6104-59-2
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein:
5718025
MDL number:
MFCD00041762
UNSPSC Code:
12171500
PubChem Substance ID:
24892085
NACRES:
NA.47

product name

Brilliant Blue R Concentrate, suitable for SDS-PAGE, methanol solution

form

methanol solution

Quality Level

200

color

dark blue

suitability

suitable for SDS-PAGE

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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Application

Brilliant Blue R concentrate is a methanol based stain especially designed for staining protein in polyacrylamide but will also stain agarose gels. The stain contains methanol and acetic acid so gels do not require fixing prior to staining.
Brilliant Blue R Concentrate has been used for protein band detection after nondenaturing gradient gel electrophoresis, isoelectric focussing and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis).
For detection of protein bands following electrophoresis.

Reconstitution

After dilution with deionized water to a final volume of one liter, solution will contain 0.25% (w/v) Brilliant Blue R, 40% (v/v) methanol and 7% (v/v) acetic acid.

Legal Information

related product

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Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1

Target Organs

Eyes

WGK

WGK 3

Flash Point(F)

57.2 °F

Flash Point(C)

14 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

危险化学品

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Crystallization of soluble proteins in vapor diffusion for x-ray crystallography.
Benvenuti M and Mangani S
Nature Protocols, 2, 1633-1651 (2007)
Overexpression of human apolipoprotein AI in transgenic rats and the hyperlipoproteinemia associated with experimental nephrosis.
Burkey BF, et al.
Journal of Lipid Research, 36.7, 1463-1473 (1995)
The effect of ethanol or hepatotoxin exposure on rat transferrin desialylation.
Lof K, et al.
Alcohol and Alcoholism (Oxford, Oxfordshire), 31, 445-451 (1996)
Katharina Stock et al.
Cancer cell international, 19, 77-77 (2019-04-13)
The nucleation-promoting factor cortactin is expressed and promotes tumor progression and metastasis in various cancers. However, little is known about the biological role of cortactin in the progression of pancreatic ductal adenocarcinoma (PDAC). Cortactin and phosphorylated cortactin (Y421) were investigated
Yong-Hak Kim et al.
Archives of biochemistry and biophysics, 494(2), 159-165 (2009-12-01)
We studied the decolorization of malachite green (MG) by the fungus Cunninghamella elegans. The mitochondrial activity for MG reduction was increased with a simultaneous increase of a 9-kDa protein, called CeCyt. The presence of cytochrome c in CeCyt protein was
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