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B0149

Sigma-Aldrich

Brilliant Blue R

Dye content ~50 %, Powder

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Synonym(s):
Acid Blue 83, Brilliant indocyanin 6B, Coomassie Brilliant Blue R
Empirical Formula (Hill Notation):
C45H44N3NaO7S2
CAS Number:
6104-59-2
Molecular Weight:
825.97
Colour Index Number:
42660
Beilstein:
5718025
EC Number:
228-060-5
MDL number:
MFCD00041762
UNSPSC Code:
12171500
PubChem Substance ID:
24891505
NACRES:
NA.47

Quality Level

200

form

powder

composition

Dye content, ~50%

solubility

H2O: 10 mg/mL

suitability

suitable for detection of protein bands following electrophoresis.

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Na+].CCOc1ccc(Nc2ccc(cc2)C(\c3ccc(cc3)N(CC)Cc4cccc(c4)S([O-])(=O)=O)=C5\C=C/C(C=C5)=[N+](\CC)Cc6cccc(c6)S([O-])(=O)=O)cc1

InChI

1S/C45H45N3O7S2.Na/c1-4-47(31-33-9-7-11-43(29-33)56(49,50)51)40-23-15-36(16-24-40)45(35-13-19-38(20-14-35)46-39-21-27-42(28-22-39)55-6-3)37-17-25-41(26-18-37)48(5-2)32-34-10-8-12-44(30-34)57(52,53)54;/h7-30H,4-6,31-32H2,1-3H3,(H2,49,50,51,52,53,54);/q;+1/p-1

InChI key

NKLPQNGYXWVELD-UHFFFAOYSA-M

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General description

Brilliant Blue R (C.l. 42660) is a triaminotriarylmethane dye with two amino groups being benzylated and one phenylated. It is also known as brilliant indocyanine 6B and coomassie blue R250.

Application

Brilliant Blue R has been widely used for protein band detection after SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis).Its other applications include:
  • as a protein stain on epoxy-embedded tissue sections
  • for quantitative micro densitometry of protein in sections
  • for staining chromatids, actinin microfilaments, developing neurites, and sperm acrosomes.
For detection of protein bands following electrophoresis.

Legal Information

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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