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Key Documents

B0519

Sigma-Aldrich

Biotin−Agarose

PBS suspension

Synonym(s):

Vitamin H Agarose

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About This Item

MDL number:
MFCD00163072
UNSPSC Code:
41106500
NACRES:
NA.56
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form

PBS suspension

Quality Level

200

extent of labeling

≥30 mg avidin per mL packed resin
2-6 μmol per mL

matrix

4% beaded agarose

matrix activation

epoxy

matrix attachment

carboxy (amide linkage); resin remains relatively uncharged over wide pH range

matrix spacer

13 atoms

application(s)

life science and biopharma

storage temp.

2-8°C

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1 of 4

This Item
SAB1300288HPA031151SAE0093
Sigma-Aldrich

44498

β-1,4-Galactosyl Transferase I human, recombinant from Saccharomyces cerevisiae

Anti-GCNT1 (center) antibody produced in rabbit IgG fraction of antiserum, buffered aqueous solution

SAB1300288

Anti-GCNT1 (center) antibody produced in rabbit

Anti-GCNT1 antibody produced in rabbit Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

HPA031151

Anti-GCNT1 antibody produced in rabbit

Beta-1,4-galactosyltransferase 1 B4GALT1 human recombinant, expressed in HEK 293 cells, 2000 units/mg protein

SAE0093

Beta-1,4-galactosyltransferase 1

Gene Information

human ... GCNT1(2650)

Gene Information

human ... GCNT1(2650)

Gene Information

human ... GCNT1(2650)

Gene Information

-

recombinant

expressed in Saccharomyces cerevisiae

recombinant

-

recombinant

-

recombinant

expressed in HEK 293 cells

concentration

≥0.2 unit/mL, 0.5-4 mg/mL protein

concentration

-

concentration

-

concentration

-

UniProt accession no.

Q02742

UniProt accession no.

Q02742

UniProt accession no.

Q02742

UniProt accession no.

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

impurities

≤0.1% β-galactosidase

impurities

-

impurities

-

impurities

-

Application

Biotin contains a high affinity for binding to avidin or streptavidin. It has been widely used in biochemistry and molecular biology for detecting and labeling proteins.

Physical form

Suspension in phosphate buffered saline containing 0.02% sodium azide

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000393799
    0000393800
    0000393799
    0000393800
    0000292851

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    P A Smith et al.
    Nucleic acids research, 26(6), 1414-1420 (1998-04-29)
    The high affinity binding interaction of biotin to avidin or streptavidin has been used widely in biochemistry and molecular biology, often in sensitive protein detection or protein capture applications. However, in vitro chemical techniques for protein biotinylation are not always
    Emrah Celik et al.
    Journal of molecular recognition : JMR, 25(1), 53-56 (2012-01-04)
    Sample-probe contact duration (dwell time) and loading force are two important parameters for the atomic force microscopy (AFM) force spectroscopy measurements of ligand-receptor interaction. A prolonged contact time may be required to initiate ligand-receptor binding as a result of slow
    Dietmar Eberbeck et al.
    Journal of nanobiotechnology, 6, 4-4 (2008-03-13)
    The binding reaction of the biomolecules streptavidin and anti-biotin antibody, both labelled by magnetic nanoparticles (MNP), to biotin coated on agarose beads, was quantified by magnetorelaxometry (MRX). Highly sensitive SQUID-based MRX revealed the immobilization of the MNP caused by the
    Biotinylated CdSe/ZnSe nanocrystals for specific fluorescent labeling.
    Charvet, N., et al.
    Journal of Materials Chemistry, 14, 2638-2642 (2004)
    K J Airenne et al.
    Gene, 144(1), 75-80 (1994-06-24)
    A recombinant avidin (re-Avd), containing amino acids (aa) 1-123 of the native chicken egg-white Avd, was produced in Escherichia coli. When cells were grown at 37 degrees C production was over 1 microgram/ml, due to altering the codon preference of
    View All Related Papers

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