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B4639

Sigma-Aldrich

Biotin

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

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Synonym(s):
D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H
Empirical Formula (Hill Notation):
C10H16N2O3S
CAS Number:
58-85-5
Molecular Weight:
244.31
Beilstein:
86838
EC Number:
200-399-3
MDL number:
MFCD00005541
UNSPSC Code:
12352201
eCl@ss:
34058011
PubChem Substance ID:
24891795
NACRES:
NA.21

product line

BioReagent

Quality Level

200

Assay

≥99%

form

powder

mol wt

Mw 244.31 g/mol

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable
cell culture | plant: suitable

color

white to off-white

mp

231-233 °C (lit.)

solubility

H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)

storage temp.

2-8°C

SMILES string

[H][C@]12CS[C@@H](CCCCC(O)=O)[C@@]1([H])NC(=O)N2

InChI

1S/C10H16N2O3S/c13-8(14)4-2-1-3-7-9-6(5-16-7)11-10(15)12-9/h6-7,9H,1-5H2,(H,13,14)(H2,11,12,15)/t6-,7-,9-/m0/s1

InChI key

YBJHBAHKTGYVGT-ZKWXMUAHSA-N

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General description

Biotin is also called as B complex vitamin. It is a bicyclic compound, which has a molecular weight of 244.31 Da. Biotin serves as an important cofactor for mammalian carboxylases. It is found within the cells in free and bound pools.

Application

Biotin has been used:
  • as a M199 media supplement in in vitro culture system
  • as a component of SATO serum free medium for the isolation of cortical oligodendrocyte precursors (OPCs)
  • in biochemical precursors culture for de novo fatty acid synthesis
  • as a component in aggregate culture media

Biochem/physiol Actions

Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.

related product

Product No.
Description
Pricing

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Biochemical precursor effects on the fatty acid production in cell suspension cultures of Theobroma cacao L.
Parra O, et al.
Plant Physiology and Biochemistry, 111, 59-66 (2017)
Preparation of rat brain aggregate cultures for neuron and glia development studies
Koito H and Li J
Journal of Visualized Experiments, 31 (2009)
Convergent functional genomics of oligodendrocyte differentiation identifies multiple autoinhibitory signaling circuits
Gobert R P, et al.
Molecular and cellular biology, 29(6), 1538-1553 (2009)
Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery
Mehta S R, et al.
Clinical and Vaccine Immunology : CVI, 15(12), 1764-1770 (2008)
Encyclopedia of dietary supplements (2010)
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Articles

Biotin in Cell Culture

Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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