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A8094

L-Arginine

Name: <SC>L</SC>-Arginine
Brand: SIGMA

98.5-101.0%, suitable for cell culture, BioXtra, non-animal source

Synonym(s):

(S)-2-Amino-5-guanidinopentanoic acid

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About This Item

Linear Formula:

H₂NC(=NH)NH(CH₂)₃CH(NH₂)CO₂H

CAS Number:

74-79-3

Molecular Weight:

174.20

Beilstein:

1725413

EC Number:

200-811-1

MDL number:

MFCD00002635

UNSPSC Code:

12352209

eCl@ss:

32160406

PubChem Substance ID:

24891316

NACRES:

NA.26

product name

L-Arginine, from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

biological source

non-animal source

Quality Level

300

Agency

USP/NF
meets EP testing specifications
meets USP testing specifications

product line

BioXtra

Assay

98.5-101.0%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

222 °C (dec.) (lit.)

solubility

H₂O: 100 mg/mL

application(s)

agriculture
peptide synthesis
pharmaceutical (small molecule)

SMILES string

N[C@@H](CCCNC(N)=N)C(O)=O

InChI

1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m0/s1

InChI key

ODKSFYDXXFIFQN-BYPYZUCNSA-N

Gene Information

human ... NOS1(4842) , NOS2(4843)
rat ... Ppm1a(24666)

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Related Categories

Amino Acids

Amino Acids, Resins & Reagents for Peptide Synthesis

High-Purity Biochemicals and Reagents

Application

L-Arginine has been used:

as a Roswell park memorial institute medium (RPMI) media component in the isolation and culture of peripheral blood mononuclear cells (PBMCs)
as a RPMI media component for tissue culture
in DMEM medium for the identification and quantification of phosphorylation sites by stable isotope labeling by amino acids in cell culture (SILAC) and LCMS/MS

Biochem/physiol Actions

L-Arginine is a conditionally essential amino acid, that play a vital role in the production of nitric oxide. It acts as a precursor for the synthesis of polyamines, agmatine and urea. L-Arginine is usually essential in children and non-essential in adults.

Substrate of nitric oxide synthase, which is converted to citrulline and nitric oxide (NO). Induces insulin release by a nitric oxide-dependent mechanism.

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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J. P. Felix D'Mello

Amino Acids in Human Nutrition and Health, 225-225 (2012)

Requirement of the mTOR Kinase for the Regulation of Maf1 Phosphorylation and Control of RNA Polymerase III-dependent Transcription in Cancer Cells

Boris Shor

The Journal of Biological Chemistry, 285(20), 15380-15392 (2010)

Association of reactive oxygen and nitrogen intermediate and complement levels with apoptosis of peripheral blood mononuclear cells in lupus patients

Oates J C, et al.

Arthritis and Rheumatism, 56(11), 3738-3747 (2007)

l-Citrulline Metabolism in Mice Augments CD4+ T Cell Proliferation and Cytokine Production In Vitro, and Accumulation in the Mycobacteria-Infected Lung

Lange S M, et al.

Frontiers in Immunology, 8, 1561-1561 (2017)

Anti-aging effects of l-arginine

Mohamed Z.Gad

Journal of Advanced Research, 1, 169-177 (2010)

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Protocols

Protocol Guide: XTT Assay for Cell Viability and Proliferation

XTT assay protocol for measuring cell viability, proliferation, activation and cytotoxicity. Instructions for XTT reagent preparation and examples of applications.

MTT Assay Protocol for Cell Viability and Proliferation

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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