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Key Documents

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Safety Information

A7089

Sigma-Aldrich

Accumax™ solution

sterile-filtered, suitable for cell culture

Synonym(s):

Cell detachment solution

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1 EA
CN¥6,218.64

CN¥6,218.64

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1 EA
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About This Item

MDL number:
MFCD01632904
UNSPSC Code:
12352204
NACRES:
NA.75

CN¥6,218.64

Available to ship onApril 27, 2025Details

Request a Bulk Order

sterility

sterile-filtered

Quality Level

200

form

solution

concentration

1 ×

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

pH

6.8-7.8

shipped in

dry ice

storage temp.

−20°C

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particle size

5 μm

particle size

3 μm

particle size

3 μm

particle size

3 μm

matrix

silica gel, high purity, spherical base material, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

matrix

silica gel, high purity, spherical particle platform, fully porous particle

separation technique

hydrophilic interaction (HILIC), reversed phase, normal phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

matrix active group

cyano phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

matrix active group

C18 (octadecyl) phase

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

technique(s)

HPLC: suitable, LC/MS: suitable

pore size

180 Å

pore size

120 Å

pore size

120 Å

pore size

120 Å

Application

Accumax has proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, COS and Sf9 cells. The solution performed extremely well in dissociating cell counting, viral transfection assays, cell sorting, flow cytometry, and bioreactor scale-up. It has also been used to create single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue, and the routine detachment of cells from standard tissue culture plasticware and adhesion coated plasticware.
TM=["Accumax"] solution has been used:
  • for detaching single walled carbon nanotubes from the extracellular matrix (ECM) in epithelial cells[1]
  • for dissociation of retinal pigmented epithelial cells prior to passaging[2]
  • for dissociation of islets from pancreas[3]

Biochem/physiol Actions

Accumax is a cell detachment solution consisting of proteolytic and collagenolytic enzymes. Each bottle has 100 mL of 1x Accumax enzymes in Dulbecco′s PBS. There are no mammalian or bacterial-derived products found in Accumax.

Features and Benefits

Ready-to-use sterile liquid for in vitro cell applications

Caution

Accumax is stable when stored at -20°C. After thawing, the product can be stored for up to 2 months at 4°C.

Formula variant

Prepared in Dulbecco′s PBS (0.2 g/L KCl, 0.2 g/L KH2PO4, 8 g/L NaCl, and 1.15 g/L Na2HPO4). Refer to Product Data Sheet on the website for product usage information.

Preparation Note

This product is supplied as a sterile, ready-to-use frozen liquid. Accumax should be thawed at 37°C before use, and added at equal volume to a sample of clumped cells. Allow 5-10 minutes at 37°C for incubation and count cells as you would normally.

Legal Information

Accumax is a trademark of Innovative Cell Technologies, Inc.

related product

Product No.
Description
Pricing

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

动植物源性产品

    • Choose from one of the most recent versions:

    Certificates of Analysis (COA)

    Lot/Batch Number
    0000350932
    0000407379
    0000406897
    0000369798
    0000407379

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    1. Which document(s) contains shelf-life or expiration date information for a given product?

      If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

    2. How do I get lot-specific information or a Certificate of Analysis?

      The lot specific COA document can be found by entering the lot number above under the "Documents" section.

    3. How do I find price and availability?

      There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

    4. What is the Department of Transportation shipping information for this product?

      Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

    5. What is the appropriate cell dissociating product to be used for ES cells that will be injected into blastocysts?

      Accutase®, product A6964, or Accumax™ (Accutase® with DNase), product A7089, will work well for dissociation of ES cells. The Accutase® product has protease and dispase activity to dissociate the cells.  The Accumax™ product also has DNase to limit the aggregation that is caused by DNA released from ruptured cells during the dissociation/scraping.Please see the reference and link below for more information on the use of Accutase® with ES cells:Mol Reprod Dev. 2008 May;75(5):818-27

    6. My question is not addressed here, how can I contact Technical Service for assistance?

      Ask a Scientist here.

    Influence of single walled carbon nanotubes at subtoxical concentrations on cell adhesion and other cell parameters of human epithelial cells
    Kaiser JP, et al.
    Journal of King Saud University - Science, 25(1), 15-27 (2013)
    Generation of retinal pigmented epithelium from iPSCs derived from the conjunctiva of donors with and without age related macular degeneration
    Geng Z, et al.
    PLoS ONE, 12(3), e0173575-e0173575 (2017)
    Benjamin T Dodsworth et al.
    Scientific reports, 10(1), 10542-10542 (2020-07-01)
    Naïve human pluripotent stem cells (hPSC) resemble the embryonic epiblast at an earlier time-point in development than conventional, 'primed' hPSC. We present a comprehensive miRNA profiling of naïve-to-primed transition in hPSC, a process recapitulating aspects of early in vivo embryogenesis.
    Chronic beta-cell depolarization impairs beta-cell identity by disrupting a network of ca2+-regulated genes
    Stancill JS, et al.
    Diabetes, 66(8), 2175-2187 (2017)
    James A Innes et al.
    Laboratory investigation; a journal of technical methods and pathology (2021-11-17)
    The phenotype of glioma-initiating cells (GIC) is modulated by cell-intrinsic and cell-extrinsic factors. Phenotypic heterogeneity and plasticity of GIC is an important limitation to therapeutic approaches targeting cancer stem cells. Plasticity also presents a challenge to the identification, isolation, and
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    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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