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C5914

Sigma-Aldrich

Cell Dissociation Solution Non-enzymatic 1x

Prepared in phosphate buffered saline without calcium and magnesium, sterile-filtered, BioReagent, suitable for cell culture

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MDL number:
MFCD00282844
UNSPSC Code:
12352204
NACRES:
NA.75

Quality Level

200

sterility

sterile-filtered

product line

BioReagent

form

liquid

concentration

1 ×

technique(s)

cell culture | mammalian: suitable

pH

6.9-7.1

shipped in

wet ice

storage temp.

2-8°C

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General description

Cell dissociation by non-enzymatic methods in human mesenchymal stromal cells aid in their detachment and preserves their functionality especially the expression of cell surface chemokine receptor.

Application

Cell Dissociation Solution Non-enzymatic 1x has been used for the dissociation of retinal astrocytes culture, U87 glioblastoma tumor cells and cardiac stem cells (CSCs).
Special non-enzymatic formulation for gently dislodging adherent cell types from plastic or glass surfaces.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. Can one use Product C5914, Cell Dissociation Solution, with tissue?

    We have only used with monolayer cell culture, and therefore we have no information on its usage with tissue.

  6. Does Product C5914, Cell Dissociation Solution, contain EDTA?

    Yes, it does contain EDTA; the concentration is proprietary.

  7. Does Product C5914, Cell Dissociation Solution, work with epithelial cells?

    It will work with most cell types.

  8. When trypsin is used to detach cells, one usually adds fetal bovine serum (FBS) to the media. Should FBS be used with Product C5914, Cell Dissociation Solution?

    No. FBS is used to inactivate trypsin. This product is not enzymatic, so there is no need to use FBS. The cells can be washed with phosphate buffered saline (PBS). When the cells are to be cultured, then one would need to add FBS.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Ineke D C Jansen et al.
International journal of molecular sciences, 21(17) (2020-08-29)
Lysosome associated membrane proteins (LAMPs) are involved in several processes, among which is fusion of lysosomes with phagosomes. For the formation of multinucleated osteoclasts, the interaction between receptor activator of nuclear kappa β (RANK) and its ligand RANKL is essential.
Non-enzymatic dissociation of human mesenchymal stromal cells improves chemokine-dependent migration and maintains immunosuppressive function
Garg A, et al.
Cytotherapy, 16(4), 545-559 (2014)
Nyla Naim et al.
Methods in molecular biology (Clifton, N.J.), 2173, 201-216 (2020-07-12)
cAMP is a crucial mediator of multiple cell signaling pathways. This cyclic nucleotide requires strict spatiotemporal control for effective function. Light-activated proteins have become a powerful tool to study signaling kinetics due to having quick on/off rates and minimal off-target
Human cardiac stem cells with reduced notch signaling show enhanced therapeutic potential in a rat acute infarction model
Matsuda T, et al.
Circulation Journal, 78(1), 222-231 (2014)
Isolation and characterization of murine retinal astrocytes
Scheef E, et al.
Molecular Vision, 11(4), 613-624 (2005)
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