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A7015

Sigma-Aldrich

N-(2-Fluorenyl)acetamide

≥98% (HPLC)

Synonym(s):

2-AAF, 2-Acetamidofluorene, N-Acetyl-2-aminofluorene

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1 L
CN¥397.95
2.5 L
CN¥599.59
4 L
CN¥1,336.23
4 L
CN¥1,420.85
10 L
CN¥2,431.86
25 L
CN¥2,845.17

CN¥397.95

Estimated to ship onApril 22, 2025Details

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1 L
CN¥397.95
2.5 L
CN¥599.59
4 L
CN¥1,336.23
4 L
CN¥1,420.85
10 L
CN¥2,431.86
25 L
CN¥2,845.17

About This Item

Empirical Formula (Hill Notation):
C15H13NO
CAS Number:
53-96-3
Molecular Weight:
223.27
Beilstein:
2807677
EC Number:
200-188-6
MDL number:
MFCD00001116
UNSPSC Code:
12352103
PubChem Substance ID:
24891166
NACRES:
NA.25

CN¥397.95

Estimated to ship onApril 22, 2025Details

Request a Bulk Order

Assay

≥98% (HPLC)

form

powder

mp

192-196 °C (lit.)

SMILES string

CC(=O)Nc1ccc-2c(Cc3ccccc-23)c1

InChI

1S/C15H13NO/c1-10(17)16-13-6-7-15-12(9-13)8-11-4-2-3-5-14(11)15/h2-7,9H,8H2,1H3,(H,16,17)

InChI key

CZIHNRWJTSTCEX-UHFFFAOYSA-N

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EZHPI

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EZHCP

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EZHGIP

人GIP（总）ELISA

assay range

accuracy: 76-103%, standard curve range: 2-200 μU/mL, linearity: 96-112%

assay range

accuracy: 88-103%, linearity: 84-120%, sensitivity: 0.5 pM, standard curve range: 2-200 pM

assay range

accuracy: 91-111%, linearity: 86-108%, standard curve range: 0.2-20 ng/mL

assay range

accuracy: 86.7%, linearity: 99.9%, sensitivity: 8.2 pg/mL, standard curve range: 8.2-2000 pg/mL

input

sample type plasma (K2 EDTA)
sample type serum
sample type cell culture supernatant

input

sample type serum
sample type plasma (K2 EDTA)

input

sample type plasma (K2 EDTA)
sample type serum

input

sample type cell culture supernatant
sample type serum
sample type plasma (K2 EDTA)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

technique(s)

ELISA: suitable

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm)

detection method

colorimetric (450nm/590nm)

Application

N-(2-Fluorenyl)acetamide (2-Acetamidofluorene, 2-AAF), a genotoxic carcinogen, is used to induce liver cancer in animal models such as the 2-AAF/partial hepatectomy rat. 2-AAF may be used to study the mechanism of liver carcinogenesis and as a reference material during its identification or quantitation.

Biochem/physiol Actions

A genotoxic carcinogen that is used to model liver carcinogenesis in rat. When N-hydroxylated by cytochrome CYP1A2 in the liver, 2-AAF forms adducts with DNA and is tumorigenic in liver and bladder.

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Danger

Hazard Statements

H302,H350

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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    Nucleotide excision repair (NER) efficiencies of DNA lesions can vary by orders of magnitude, for reasons that remain unclear. An example is the pair of N-(2'-deoxyguanosin-8-yl)-2-aminofluorene (dG-C8-AF) and N-(2'-deoxyguanosin-8-yl)-2-acetylaminofluorene (dG-C8-AAF) adducts that differ by a single acetyl group. The NER
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