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Merck
CN

S9811

S-Gal®

reagent for selection of recombinant bacterial clones

Synonym(s):

3,4-Cyclohexenoesculetin β-D-galactopyranoside

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About This Item

Empirical Formula (Hill Notation):
C19H22O9
CAS Number:
182805-65-8
Molecular Weight:
394.37
UNSPSC Code:
12352200
PubChem Substance ID:
329824635
NACRES:
NA.85

Key Documents

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Product Name

S-Gal®, reagent for selection of recombinant bacterial clones

SMILES string

OC[C@H]1O[C@@H](Oc2cc3OC(=O)C4=C(CCCC4)c3cc2O)[C@H](O)[C@@H](O)[C@H]1O

InChI

1S/C19H22O9/c20-7-14-15(22)16(23)17(24)19(28-14)27-13-6-12-10(5-11(13)21)8-3-1-2-4-9(8)18(25)26-12/h5-6,14-17,19-24H,1-4,7H2/t14-,15+,16+,17-,19-/m1/s1

InChI key

HDJJDRXZHJRVGA-DIKXUDHVSA-N

grade

Molecular Biology

sterility

non-sterile

assay

≥95% (HPLC)

form

powder

solubility

DMF: 50 mg/mL

suitability

suitable for β-galactosidase test

storage temp.

room temp

Quality Level

100

General description

S-Gal® is an autoclavable, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-Gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Other Notes

Autoclavable, microwavable growth medium complete with S-Gal and IPTG.
The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®
formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.

Preparation Note

Stock solutions of S-Gal® can be made by dissolving 50mg/ml in dimethyl formamide (DMF) and storing at -20C. Add S-Gal® (300 mg/L from stock solution ) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.

Legal Information

S-GAL is a registered trademark of Merck KGaA, Darmstadt, Germany

Application

S-Gal is a patented autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.
Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. S-Gal® is autoclavable and can be added to bacterial broth containing agar prior to autoclaving.

Biochem/physiol Actions

When S-Gal® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.

Features and Benefits

  • More intense color contrast than X-gal
  • Excellent for use in automated colony counters
  • Autoclavable for easiest use
  • No need to make stock solutions
When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal is autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

涉药品监管产品
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Certificates of Analysis (COA)

Lot/Batch Number
STBK1925
STBH0204
STBG9250
STBF6542V
STBF5545V

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