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Merck
CN

B2904

Bluo-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal

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About This Item

Empirical Formula (Hill Notation):
C14H16BrNO6
CAS Number:
97753-82-7
Molecular Weight:
374.18
UNSPSC Code:
12352200
NACRES:
NA.85
PubChem Substance ID:
24891417
MDL number:
MFCD00063691
Beilstein/REAXYS Number:
1550140

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InChI

1S/C14H16BrNO6/c15-6-1-2-8-7(3-6)9(4-16-8)21-14-13(20)12(19)11(18)10(5-17)22-14/h1-4,10-14,16-20H,5H2/t10-,11+,12+,13-,14-/m1/s1

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

InChI key

LINMATFDVHBYOS-MBJXGIAVSA-N

grade

Molecular Biology

sterility

non-sterile

assay

≥98% (HPLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

Quality Level

200

Related Categories

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Biochem/physiol Actions

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000516229
0000516229
0000516228
0000480368
0000480368

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