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Safety Information

B2904

Sigma-Aldrich

Bluo-Gal

reagent for selection of recombinant bacterial clones

Synonym(s):

5-Bromo-3-indolyl β-D-galactopyranoside, substrate for β-galactosidase, Bluo-Gal

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200 MG
¥3,011.27

¥3,011.27

Available to ship on2025年4月14日Details

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200 MG
¥3,011.27

About This Item

Empirical Formula (Hill Notation):
C14H16BrNO6
CAS Number:
97753-82-7
Molecular Weight:
374.18
Beilstein:
1550140
MDL number:
MFCD00063691
UNSPSC Code:
12352200
PubChem Substance ID:
24891417
NACRES:
NA.85

¥3,011.27

Available to ship on2025年4月14日Details

grade

Molecular Biology

Quality Level

200

sterility

non-sterile

Assay

≥98% (HPLC)

form

powder

technique(s)

nucleic acid detection: suitable

solubility

DMF: soluble

suitability

suitable for substrate for β-galactosidase

storage temp.

−20°C

SMILES string

OC[C@H]1O[C@@H](Oc2c[nH]c3ccc(Br)cc23)[C@H](O)[C@@H](O)[C@H]1O

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PHR1834

Itraconazole

Itraconazole ≥98% (HPLC)

I6657

Itraconazole

Imidazole Pharmaceutical Secondary Standard; Certified Reference Material

PHR1180

Imidazole

Iminostilbene Pharmaceutical Secondary Standard; Certified Reference Material

PHR1197

Iminostilbene

format

neat

format

-

format

neat

format

neat

Quality Level

300

Quality Level

200

Quality Level

300

Quality Level

300

grade

certified reference material, pharmaceutical secondary standard

grade

-

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

storage temp.

2-30°C

storage temp.

2-8°C

storage temp.

2-30°C

storage temp.

2-30°C

packaging

pkg of 200 mg

packaging

-

packaging

-

packaging

-

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis,[1] Dictyostelium samples[2] and in the staining of vena cava of adult mice.[3]
Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

监管及禁止进口产品

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    0000396640
    0000396641
    0000387702

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