Skip to Content

Dear Customer:

The current international situation is complex and volatile, and uncertain tariff policies may potentially impact our product prices. Given these uncertainties, we value your understanding regarding order-related matters.

If you decide to place an order during this period, we reserve the right to adjust the price based on the evolving situation. We understand that market changes may cause inconvenience. We will negotiate with you if there’s a significant price fluctuation due to tariff policy changes before the order’s actual delivery, and in such cases we may adjust or cancel the order as necessary.

We are planning system maintenance between Friday, Apr 11 at 9:00 PM CDT and Saturday, Apr 12 at 9:00 AM CDT. This will impact both web and offline transactions, including online orders, quotes, price and availability checks, and order status inquiries. We apologize for any inconvenience.

Merck
CN
All Photos(4)

Key Documents

Safety Information

CMC0016

Sigma-Aldrich

BL21(DE3) Electrocompetent Cells

Escherichia coli, rod shaped

Synonym(s):

BL21 strain

Sign Into View Organizational & Contract Pricing

Select a Size

20 EA
CN¥3,228.10

CN¥3,228.10

Please contact Customer Service for Availability
All Photos(4)

Select a Size

Change View
20 EA
CN¥3,228.10

About This Item

UNSPSC Code:
41106202
NACRES:
NA.85

CN¥3,228.10

Please contact Customer Service for Availability

Product Name

BL21(DE3) Electrocompetent Cells, for protein expression

biological source

Escherichia coli

grade

Molecular Biology

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: electrocompetent
transformation efficiency: ≥5 × 109 cfu/μg

shipped in

dry ice

storage temp.

−70°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAB5200764SAB5200775SAB5200772
Anti-Stargazin Antibody Chemicon®, from rabbit

AB9876

Anti-Stargazin Antibody

Monoclonal Anti-Tarp Gamma2/4/8 (Stargazin) - Atto 488 antibody produced in mouse clone S245-36, purified immunoglobulin

SAB5200764

Monoclonal Anti-Tarp Gamma2/4/8 (Stargazin) - Atto 488 antibody produced in mouse

Monoclonal Anti-Tarp Gamma2/4/8 (Stargazin) - Hrp antibody produced in mouse clone S245-36, purified immunoglobulin

SAB5200775

Monoclonal Anti-Tarp Gamma2/4/8 (Stargazin) - Hrp antibody produced in mouse

Monoclonal Anti-Tarp Gamma2/4/8 (Stargazin) - Apc antibody produced in mouse clone S245-36, purified immunoglobulin

SAB5200772

Monoclonal Anti-Tarp Gamma2/4/8 (Stargazin) - Apc antibody produced in mouse

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

species reactivity

rat, mouse

species reactivity

rat, mouse

species reactivity

rat, mouse

species reactivity

mouse, rat

clone

polyclonal

clone

S245-36, monoclonal

clone

S245-36, monoclonal

clone

S245-36, monoclonal

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

General description

The BL21(DE3) Electrocompetent Cells are the first to offer high efficiency cloning and high level protein expression in the same cell.
Cloning efficiencies are increased 25-1,000 fold relative to other preparations of BL21 cells, which is essential for construction of complex expression libraries.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

Features and Benefits

The unprecedented transformation efficiency of the BL21(DE3) Electrocompetent Cells (> 5 × 109 cfu/μg) eliminates the need for plasmid transfer from the cloning strain to the expression strain, saving days of work in a typical cloning and expression experiment

Components

  • BL21(DE3) electrocompetent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


Storage Class Code

10 - Combustible liquids

Regulatory Information

新产品

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCM4783
SLCP1550
SLCM4784
SLCJ4196
SLCJ5997

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Julianne M Troiano et al.
eLife, 10 (2021-01-16)
Under high light, oxygenic photosynthetic organisms avoid photodamage by thermally dissipating absorbed energy, which is called nonphotochemical quenching. In green algae, a chlorophyll and carotenoid-binding protein, light-harvesting complex stress-related (LHCSR3), detects excess energy via a pH drop and serves as
Felix Nicolaus et al.
eLife, 10 (2021-02-09)
We follow the cotranslational biosynthesis of three multispanning Escherichia coli inner membrane proteins in vivo using high-resolution force profile analysis. The force profiles show that the nascent chain is subjected to rapidly varying pulling forces during translation and reveal unexpected
Zev A Ripstein et al.
eLife, 9 (2020-01-10)
The ClpXP degradation machine consists of a hexameric AAA+ unfoldase (ClpX) and a pair of heptameric serine protease rings (ClpP) that unfold, translocate, and subsequently degrade client proteins. ClpXP is an important target for drug development against infectious diseases. Although
Salvatore Di Girolamo et al.
Microbial cell factories, 19(1), 170-170 (2020-08-29)
Miniaturization of biochemical reaction volumes within artificial microcompartments has been the key driver for directed evolution of several catalysts in the past two decades. Typically, single cells are co-compartmentalized within water-in-oil emulsion droplets with a fluorogenic substrate whose conversion allows
Brandon R Lowe et al.
eLife, 10 (2021-02-02)
Sequencing of cancer genomes has identified recurrent somatic mutations in histones, termed oncohistones, which are frequently poorly understood. Previously we showed that fission yeast expressing only the H3.3G34R mutant identified in aggressive pediatric glioma had reduced H3K36 trimethylation and acetylation
View All Related Papers

Protocols

BL21(DE3) Electrocompetent Cells

Efficient BL21(DE3) electrocompetent cell transformation protocol ensures optimal settings for high efficiency.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service