AB9876
Anti-Stargazin Antibody
Chemicon®, from rabbit
Synonym(s):
Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)
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100 μG
CN¥6,710.12
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100 μG
CN¥6,710.12
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
1 of 4
This Item | SAB5200764 | SAB5200775 | SAB5200772 |
|---|---|---|---|
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| antibody form affinity isolated antibody | antibody form purified immunoglobulin | antibody form purified immunoglobulin | antibody form purified immunoglobulin |
| species reactivity rat, mouse | species reactivity rat, mouse | species reactivity rat, mouse | species reactivity mouse, rat |
| clone polyclonal | clone S245-36, monoclonal | clone S245-36, monoclonal | clone S245-36, monoclonal |
| shipped in dry ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
Specificity
Stargazin [Stargazin-like TARP (transmembrane AMPA receptor regulatory protein)]. By Western blot the antibody recognizes bands at ~35 kDa and ~55 kDa on Triton soluble fraction of rat brain. The antibody reacts dominantly with TARP gamma-8 and weakly with gamma-3 and gamma-4. Stargazin is expressed strongly in the hippocampus and cerebellum.
Immunogen
GST fusion protein from rat stargazin, cytoplasmic domain.
Application
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
This Anti-Stargazin Antibody is validated for use in IP, WB, IC, IH for the detection of Stargazin.
Western blot: 0.1 μg/mL on Triton soluble fraction of rat brain. Suggested dilution buffer is TBS-T and blocking buffer is 3% skim milk. Suggested incubation time is 3 hours to overnight at room temperature.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 0.25-0.5 μg/mL on 4% paraformaldehyde fixed tissue. Suggested dilution buffer is PBS-T. Suggested permeabilization method is 0.1% Triton X-100. Suggested incubation time is 2 hours to overnight at room temperature.
Immunocytochemistry
Immunoprecipitation: 1-2 μg. Suggested reaction volume is 200-1000 μL. Suggested total protein concentration is 2 mg/mL. Suggested incubation time is 2 hours at 2-8°C. Suggested capture agent is Protein A.
Optimal working dilutions must be determined by end user.
Physical form
Format: Purified
ImmunoAffinity Purified
Liquid in 130 mM Tris-Cl, 87 mM Glycine, pH 7.4.
Storage and Stability
Maintain at -80°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze thaw cycles. The antibody may be kept at 2-8°C for 1-3 weeks after thawing.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Serine racemase regulated by binding to stargazin and PSD-95: potential N-methyl-D-aspartate-?-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (NMDA-AMPA) glutamate neurotransmission cross-talk.
Ma, TM; Paul, BD; Fu, C; Hu, S; Zhu, H; Blackshaw, S; Wolosker, H; Snyder, SH
The Journal of Biological Chemistry null
Aichurok Kamalova et al.
Cell reports, 32(3), 107921-107921 (2020-07-23)
The anterior thalamus (AT) is critical for memory formation, processing navigational information, and seizure initiation. However, the molecular mechanisms that regulate synaptic function of AT neurons remain largely unexplored. We report that AMPA receptor auxiliary subunit GSG1L controls short-term plasticity
Ahmad H Alhowail et al.
Heliyon, 7(7), e07456-e07456 (2021-07-24)
Doxorubicin (Dox) is a chemotherapeutic agent used widely to treat a variety of malignant cancers. However, Dox chemotherapy is associated with several adverse effects, including "chemobrain," the observation that cancer patients exhibit through learning and memory difficulties extending even beyond
Xue-Yan He et al.
Proceedings of the National Academy of Sciences of the United States of America, 113(38), E5645-E5654 (2016-09-08)
AMPA-type glutamate receptors (AMPARs) mediate fast excitatory neurotransmission and predominantly assemble as heterotetramers in the brain. Recently, the crystal structures of homotetrameric GluA2 demonstrated that AMPARs are assembled with two pairs of conformationally distinct subunits, in a dimer of dimers
Balakrishnan Selvakumar et al.
Neuropharmacology, 77, 28-38 (2013-09-17)
Behavioral sensitization to cocaine is associated with increased AMPA receptor (AMPAR) surface expression in the nucleus accumbens (NAc). This upregulation is withdrawal-dependent, as it is not detected on withdrawal day (WD) 1, but is observed on WD7-21. Its underlying mechanisms
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