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Safety Information

50727

Sigma-Aldrich

Epoxide Hydrolase

≥0.13 U/mg

Synonym(s):

Hydratase, epoxide

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About This Item

CAS Number:
Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204

recombinant

expressed in E. coli

form

powder
powder with small lumps

specific activity

≥0.13 U/mg

storage temp.

2-8°C

Application

Epoxide hydrolase is used to study drug metabolism. It allows the excretion of various drugs from the body. Epoxside hydrolase is used to study DRESS syndrome, which results from a lack of the enzyme, and is also used to develop novel inhibitors . Product 50727 is used to hydrolyze p-nitrostyrene.

Biochem/physiol Actions

Epoxide hydrolase converts epoxides to trans-dihydroldiols, which are excreted from the body .

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Unit Definition

1 U corresponds to the amount of enzyme which hydrolizes 1μmol p-nitrostyrene per minute at pH 7.2 and 25°C

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Bichitra K Biswal et al.
Journal of molecular biology, 381(4), 897-912 (2008-07-01)
Mycobacterium tuberculosis (Mtb), the intracellular pathogen that infects macrophages primarily, is the causative agent of the infectious disease tuberculosis in humans. The Mtb genome encodes at least six epoxide hydrolases (EHs A to F). EHs convert epoxides to trans-dihydrodiols and
Ana W Y Cheong et al.
Biology of reproduction, 81(1), 126-132 (2009-03-27)
Somatic cell-embryo coculture enhances embryo development in vitro by producing embryotrophic factor(s) and/or removing harmful substances from the culture environment. Yet, the underlying molecular mechanisms on how somatic cells remove the toxicants from the culture medium remain largely unknown. By
Zhuo Shao et al.
Arteriosclerosis, thrombosis, and vascular biology, 34(3), 581-586 (2014-01-25)
Regulation of angiogenesis is critical for many diseases. Specifically, pathological retinal neovascularization, a major cause of blindness, is suppressed with dietary ω3-long-chain polyunsaturated fatty acids (ω3LCPUFAs) through antiangiogenic metabolites of cyclooxygenase and lipoxygenase. Cytochrome P450 epoxygenases (CYP2C8) also metabolize LCPUFAs
Gary P Carlson
Journal of toxicology and environmental health. Part A, 74(6), 347-350 (2011-01-29)
Styrene is hepatotoxic and pneumotoxic in mice. Styrene oxide, the active metabolite, is detoxified via hydrolysis by microsomal epoxide hydrolase (mEH). Racemic styrene oxide was previously found to be more lethal and produced increased toxicity in mEH-/- mice compared to

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