L2157
Laccase from Rhus vernicifera
crude acetone powder, ≥50 units/mg solid
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form
crude acetone powder
Quality Level
specific activity
≥50 units/mg solid
greener alternative product characteristics
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storage temp.
−20°C
InChI
1S/C9H13NO/c1-4-10-7(2)5-9(6-11)8(10)3/h5-6H,4H2,1-3H3
InChI key
NWDZDFOKSUDVJV-UHFFFAOYSA-N
Related Categories
General description
Unit Definition
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
Regulatory Information
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How stable are solutions of Product L2157, Laccase from Rhus vernicifera?
Solutions should be prepared fresh, directly before use, as they are not stable as stock solutions.
My solution of Product L2157, Laccase from Rhus vernicifera, isn't clear. Is this normal?
Yes. L2157 is a crude acetone powder, and can contain other proteins, enzymes, and cellular debris. A suspension may be prepared in 100 mM potassium phosphate buffer, pH 6.5, by homogenization with a hand-held homogenizer for 10 minutes. This preparation may then be centrifuged; the laccase activity will be present in the supernatant.
What is the molecular weight (MW) of Product L2157, Laccase from Rhus vernicifera?
Spira-Solomon and Solomon, J. Am. Chem. Soc., 109, 6421-6432 (1987) reports estimated molecular weights of 52 kDa (laccase 1), 55 kDa (laccase 2), and 70 kDa (laccase 3), as determined by gel filtration.
What is the pH optimum of Product L2157, Laccase from Rhus vernicifera?
Our own assay is performed at pH 6.5. One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives pH 6.5 as another value for the laccase from this species, with a range for enzyme activity of 4.3 to 9.
What is the temperature optimum of Product L2157, Laccase from Rhus vernicifera?
Our own assay is performed at 30°C. One publication (Casella et al., J. Inorg. Biochem., 100, 2127-2139 (2006)) gives 25°C as another value for the same enzyme from the same species.
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Protocols
Laccase, a biocatalyst, oxidizes aromatic compounds with molecular oxygen reduction, making it useful in various applications.
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