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Safety Information

E7404

Sigma-Aldrich

Microsomal Epoxide Hydrolase

from human, recombinant, expressed in human lymphoblast cell line

Synonym(s):

Epoxide Hydratase, Xenobiotic Epoxide Hydrolase, mEH

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About This Item

Enzyme Commission number:
UNSPSC Code:
12352204

biological source

human

recombinant

expressed in human lymphoblast cell line

form

buffered aqueous solution (100 mM potassium phosphate, pH 7.4)

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... EPHX1(2052)

Application

Microsomal epoxide hydrolase has been used in a study to assess its activation of microsomal epoxide hydrolase by interaction with cytochromes P450. Microsomal epoxide hydrolase has also been used in a study to investigate the possible role of its gene polymorphism as a risk factor for developing insulin resistance and type 2 diabetes mellitus.

Other Notes

Epoxide hydrolase functions to render epoxides less chemically reactive. Two forms of human hydrolase are involved in this process, microsomal epoxide hydrolase (mEH) and soluble epoxide hydrolase. mEH is an enzyme of the endoplasmic reticulum. Although typically expressed in the liver, it is also present at significant levels in other tissues such as the adrenal gland. The primary characteristics of mEH substrates include marked lipophilicity and a lack of any trans-substitutions on the oxirane ring.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Activation of microsomal epoxide hydrolase by interaction with cytochromes P450: kinetic analysis of the association and substrate-specific activation of epoxide hydrolase function.
Taura Ki K, Yamada H
Archives of Biochemistry and Biophysics, 402(2), 275-280 (2012)
Chen-Yang Duan et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 35(1), 659-666 (2013-08-21)
Previous studies have focused on the association of a gene (EPHX1) encoding microsomal epoxide hydrolase with the carcinogenesis of hepatocellular carcinoma (HCC). In the present study, we performed a meta-analysis to systematically summarize the possible association between EPHX1 genetic polymorphisms
Possible role of microsomal epoxide hydrolase gene polymorphism as a risk factor for developing insulin resistance and type 2 diabetes mellitus.
Ghattas, M. and M. Amer.
Endocrine J., doi :10-doi :10 (2012)
Rakesh Ghosh et al.
Mutation research, 749(1-2), 80-86 (2013-05-08)
Studies have reported gene-by-environment interaction for chronic respiratory conditions but none on acute illnesses in children. We investigated, longitudinally, whether genotype modifies the relationship of environmental exposures (second-hand tobacco smoke, polycyclic aromatic hydrocarbons, particulate matter <2.5μm (PM2.5)) with acute bronchitis
Danuta Mielzynska-Svach et al.
Mutagenesis, 28(5), 591-599 (2013-07-23)
This article is a follow-up to our previous molecular epidemiology studies on the DNA damage in children from the Upper Silesia region of Poland. It is expected that metabolic and DNA repair gene polymorphisms may modulate individual susceptibility to environmental

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